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Arp2/3 protein complex - 2x 50 µg

Cat. #:
8413-01
Shipping:
FedEx Express (Global) FedEx Express (Global) (shipping info)
Quantity:
2x50 µg
 


Arp2/3 protein complex
(Actin Binding Protein)


Cat. #: 8413-01

.....................................................................................................................................................................................................................................................................................................................................................................................................................................................

 

Description

 

 

Protein

Arp2/3 complex

Origin

brain, porcine

Molecular mass

224kDa

Protein description

The seven-subunit protein complex comprises Arp2 and Arp3 and five smaller (Arc) proteins. Both Arp2 and Arp3 sequences possess nucleotide-binding and divalent cation-binding sites. Arp 2/3 complex consists of five associated proteins to Arp2 and Arp3, namely p16, p20, p21, p34, and p41 (Synonyms: ARPC-5, -4, -3, -2, and -1). 

Arp2/3 is a constitutively inactive complex. Nucleation is efficiently activated in vitro by binding of the VCA-domains of members of the Wiskott-Aldrich syndrome family protein, like WAVE (WASP family verprolin homologous). 

Actin interaction

Pointed end capping protein. Branching of actin filaments. Strong Nucleation upon activation by VCA-domain (Cat. #: 8416-01)  




Properties

 

 

Form

Lyophilized, ready-to-use.

Quantity per unit

2x 50 µg

Buffer

150mM KCl, 20mM Trizma pH 7.5, 1mM DTT, 0.2mM ATP, 0.5mM EDTA, 1mM MgCl2 and 5% disaccharides, when reconstituted with 50µl ultrapure water to obtain a 1 mg/ml solution.

Purity

>98% by scanning densitometry from Coomassie G-250 stained SDS-Gels.

Purification notes

Purified from porcine brain.

Protein concentration

Determined at OD280 (0.1%=1.033).

Storage instructions

Arp2/3 complex is stored at –70°C upon arrival will be stable in performance for at least 6 months from the date of purchase. The solubilized Arp2/3 complex is kept on ice and should be stored at -20°C in glycerol or CryoProtect (Cat.#: 6011-01).  Avoid repeated freeze / thaw cycles. 

Shipping conditions 

At ambient temperature. Upon delivery store at -70°C.

Remarks

For Use in Research only. Not for Use in Human or Veterinary Diagnostical or Therapeutical Applications. 

CAS no.

 

 

 

 

Further Information


Product DataSheet

Material and Safety Data Sheet

Protein Sequence on NCBI (All chain sequences)

Protein Structures on RCSB


 

References
 

Initiation of DNA replication requires actin dynamics and formin activity.
Parisis N, Krasinska L, Harker B, Urbach S, Rossignol M, Camasses A, Dewar J, Morin N, Fisher D.
EMBO J. 2017 Nov 2;36(21):3212-3231. doi: 10.15252/embj.201796585. Epub 2017 Oct 5.

 

Polarized Exocytosis Induces Compensatory Endocytosis by Sec4p-Regulated Cortical Actin Polymerization.
Johansen J, Alfaro G, Beh CT.
PLoS Biol. 2016 Aug 15;14(8):e1002534. doi: 10.1371/journal.pbio.1002534. eCollection 2016 Aug.

 

Crystal structure of Arp2/3 complex.
Robinson RC. Turbedsky K. Kaiser DA. MarchandJB, Higgs HN, Choe S, Pollard TD.
Science. 2001 Nov 23;294(5547):1679-84.
 

Crystal structures of actin-related protein 2/3 complex with bound ATP or ADP.
Nolen BJ, Littlefield RS, Pollard TD.
Proc Natl Acad Sci U S A. 2004 Nov 2;101(44):15627-32. Epub 2004 Oct 25.


 

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