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Actin (porcine skeletal muscle alpha-actin) - 1.0mg

Cat. #:
8111-01
Shipping:
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Actin
alpha-actin from porcine skeletal muscle


Cat. #: 8111-01  

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Description

 

 

Protein

Actin (α-Actin)

Origin

Skeletal muscle alpha actin, porcine.

Molecular mass

42kDa

Protein description

Actin from rabbit skeletal muscle is a single chain polypeptide with a molecular weight of 42kD consisting of 375 amino acids.  It is characterized by binding to ATP/ADP (ATPase function) and the binding sites for divalent cations, that strongly contribute to the stabilization of actin.
Actin truly is a multi-ligand protein, which simply reflects its central importance as a major component of the cytoskeleton. The skeletal muscle actin (alpha-actin isoform) is highly conserved among vertebrates, which makes the rabbit skeletal muscle a commonly used protein to investigate protein-protein interactions with cytoskeletal ligands.
The ability of actin monomers e.g. to polymerize into filaments of several µm lengths, to branch or perform the typical actin kinetics, strongly depends on the quality of the actin. Hypermol is manufacturing a premium quality actin.  And to provide you the best actin we can possibly make, our proprietary freeze-drying technique conserves highly functional actin. to arrive directly at your bench. 

Purity

>99% by scanning densitometry.



Properties

 

 

Form

Lyophilized, ready-to-use.

Quantity per unit

1 mg

Buffer

2mM Tris-Cl pH 8.2, 0.4mM ATP, 0.5mM DTT, 0.1mM CaCl2, 1mM NaN3 and 0.3% disaccharides, when reconstituted with 1.0 ml ultrapure water to obtain a 1mg/ml solution.

Related products

For dilution of G-actin or exchange of ligand buffer into actin compatible buffer, MonoMix (Cat. #.: 5100-0*) may be used. In the absence of nucleators, the polymerization of G-actin is initiated by PolyMix (Cat.#: 5000-0*). 

Purification notes

Purified from porcine skeletal muscle. GPC.

Protein concentration

Determined by the Biuret method.

Storage instructions

Actin stored at –70°C upon arrival will be stable in performance for at least 6 months from the date of purchase. Solubilized G-actin is kept on ice and should be used within 1 week. Avoid refreezing.     

Shipping conditions 

At ambient temperature. Upon delivery store at -70°C.

Remarks

For Use in Research only. Not for Use in Human or Veterinary Diagnostical or Therapeutical Applications.

CAS no.

 51005-14-2

 

 
 

Further Information



Product DataSheet


Material and Safety Data Sheet


Protein Sequence on NCBI

 





References

 

Cosolvent and crowding effects on the polymerization kinetics of actin.
Rosin, Christopher, Paul Hendrik Schummel and Robert S. C. Winter.

 Physical chemistry chemical physics : PCCP 17 13 (2015): 8330-7.

 

Exploring the stability limits of actin and its suprastructures.
Rosin C, Erlkamp M, Ecken JV, Raunser S, Winter R.

Biophys J. 2014 Dec 16;107(12):2982-2992. doi: 10.1016/j.bpj.2014.11.006.

 

Multivalent cross-linking of actin filaments and microtubules through the microtubule-associated protein Tau.
Cabrales Fontela Y, Kadavath H, Biernat J, Riedel D, Mandelkow E, Zweckstetter M.

Nat Commun. 2017 Dec 7;8(1):1981. doi: 10.1038/s41467-017-02230-8.



 

 

 
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