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CapZ (non-muscle, human recombinant) - 2x50 µg

Cat. #:
8322-01
Shipping:
FedEx Express (Global) FedEx Express (Global) (shipping info)
Quantity:
2x50 µg
Units:
1x


Cap Z (capping protein)
(Actin Binding Protein)


Cat. #: 8322-01 

.....................................................................................................................................................................................................................................................................................................................................................................................................................................................

 
Description

 

 

Protein

Cap Z, Capping protein (non-muscle isoform)

Origin

recombinant, human recombinant

Molecular mass

66kDa

Protein description

The subunits of the heterodimeric capping protein CapZ possess a molecular mass of 34kDa (alpha 1-subunit) and 32kDa (beta 2-subunit, His-tagged). CapZ binds with high affinity in a Ca++-independent manner to the barbed end of actin filaments (1:1000 M/M).

Actin interaction

Ca++-insensitive barbed end capping protein. Kd actin binding = >1nM (barbed end) .

 

Properties

 

 

Form

Lyophilized, ready-to-use.

Quantity per unit

2x50µg

Buffer

150mM NaCl, 20mM Hepes pH 7.4, 1mM DTT and 5% sucrose, when reconstituted with 50 µl ultrapure water to obtain a 1.0 mg/ml solution.

Purity & Activity


Purity: >94% by scanning densitometry from Coomassie G-250 stained SDS-Gels.

Capping activity: F-actin (Cat.#:8101) containing 20% pyrene actin (Cat.#: 8112) was incubated with CapZ and diluted below the critical concentration. Depolymerisation was followed by fluorometry and compared to an actin reference without CapZ.

Complete capping was obtained at a molar ratio of 1 : 1000 (CapZ : Actin). All proteins were used as supplied, i.e. without dialysis. G-Actin stock solutions were pre-spun 1h, 100.000xg, 4°C.  

Purification notes

The alpha-1 SU is untagged, while the beta-2 SU contains a His-tag. Using anti-His antibodies, the protein can be identified by all suited immunological  techniques.

Protein concentration

Determined by OD280 (0.1%=1.234).

Storage instructions

CapZ is stored at –70°C upon arrival will be stable in performance for at least 6 months from the date of purchase. The solubilized protein is kept on ice and should be stored at -20°C in glycerol or CryoProtect (Cat.#: 6011-01). Avoid repeated freeze / thaw cycles. 

Shipping conditions 

At ambient temperature. Upon delivery store at -70°C.

Remarks

For Use in Research only. Not for Use in Human or Veterinary Diagnostical or Therapeutical Applications.

CAS no.

 

 

 

Further Information

Product DataSheet

Material and Safety Data Sheet

Sequence on NCBI (alpha-1 SU)

Sequence on NCBI (beta-2 SU)


Structure on NCBI (PDB ID: 1IZN)



 


References


Myosin-II activity generates a dynamic steady state with continuous actin turnover in a minimal actin cortex
Sonal, Kristina A. Ganzinger, Sven K. Vogel, Jonas Mücksch, Philipp Blumhardt and Petra Schwille
bioRxiv preprint first posted online May. 2, 2018; doi: https://dx.doi.org/10.1101/312512

 
Yamashita A, Maeda K, Maeda Y
Embo J. (2003) 22 p.1529
 
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