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ActinStaining Kit 488-Phalloidin

Cat. #:
8812-01
Shipping:
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ActinStaining Kit 488-Phalloidin


Cat. #: 8812-01  

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Applications

Rapid staining of single actin filaments with highly photostable 488-Phalloidin.


Description

 

 

Product

488-Phalloidin (Atto 488 phalloidin)

Origin

Amanita Phalloides Conjugate

Molecular mass

N/A

Product description

Rapid staining of single actin filaments with highly photostable 488-Phalloidin. Phalloidin is a bicyclic peptide of a family of toxins isolated from the Amanita phalloides “death cap” mushroom. The peptide is commonly used as a selective stain or counterstain to specifically label F-actin in fixed or permeabilized cells, and cell-free experiments. Labeled phalloidin conjugates bind in a stoichiometric ratio of about one phallotoxin per actin subunit in both muscle and non-muscle cells, and have similar affinity for both large and small filaments. Phalloidins do not bind to monomeric actin and have a stabilizing effect on F-actin. 

Alexa Fluor® 488 provides green fluorescence of unparalleled brightness and photostability

Actin interaction

Selective staining of actin filaments in vitro.



Properties

 

 

Form

Dry

Quantity

1x 100 Units (sufficient for 100 slides)

Buffer

Reconstitute in 100µl MeOH, DMSO or DMF. Dilute 5µl of the phalloidin stock with 500µl PBS and apply to stain the sample.

Sub cellular localization

Actin, Cytoskeleton

Excitation/Emission

ex 488 nm, em 523 nm

Kit Content

1x 100 Units 488-Phalloidin;
1x PolyMix (10x conc., F-actin buffer, lyophilized); 

Storage instructions

Upon reconstitution the product is stored at -5 to -70°C. Always protect from light.

Shipping conditions 

At ambient temperature. Upon delivery store at -5 to -70°C.

Remarks

 

CAS no.

 N/A

 



Further Information


Table of Dyes and Light Sources