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ATTO550-Actin (non-muscle actin) - 2x50µg

Cat. #:
8355-01
Shipping:
FedEx Express (Global) FedEx Express (Global) (shipping info)
Quantity:
2x50 µg

 


ATTO550-Actin
Non-muscle actin from brain, porcine


Cat. #: 8355-01
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Applications

Actin assays for epifluorescence. high and superresolution microscopy, FRET, biochemical actin assays.

 

Description

 

 

Protein

ATTO550-Actin

Origin

Non-muscle  β/γ-actin, porcine

Molecular mass

42kDa

Protein description

Actin from non-muscle sources is a mixture of  β/γ non-muscle actin isoforms, Both are single chain polypeptides with a molecular weight of 42kD consisting of 374 amino acids. ATTO550-Actin is chemically modified G-actin with an ATTO550 fluorescent group covalently bound to lysine residues. ATTO550-Actin (λex 550nm, λem 576nm) possesses the polymerization properties of native G-actin. 

Conjugation ratio

This product is supplied with a degree of labelling (DOL) of 0.5-0.8. 

 

Properties

 

 

Form

Lyophilized, ready-to-use.

Quantity  per unit

2x50 µg

Buffer

2mM Tris-Cl pH 8.2, 1.0mM ATP, 0.5mM DTT, 0.1mM CaCl2, 1mM NaN3 and 0.3% disaccharides, when reconstituted with 100 µl ultrapure water to obtain a 1mg/ml solution.

Purity

>99% by scanning densitometry.

Purification notes

Purified by GPC from porcine brain

Protein concentration

Determined by the Biuret method.

Storage instructions

ATTO550-Actin is stored at –70°C upon arrival and will be stable in performance for at least 6 months from the date of purchase. Solubilized ATTO-Actin is kept on ice and should be used within 1 week. Avoid refreezing.   

Shipping conditions 

At ambient temperature. Upon delivery store at -70°C.

Remarks

For Use in Research only. Not for Use in Human or Veterinary Diagnostical or Therapeutical Applications.

CAS no.

 

 

 

Further Information


Product DataSheet

Material and Safety Data Sheet

Table of Dyes and Light Sources 

Protein Sequence on NCBI