ATTO594-Actin (non-muscle actin) - 2x50µg
Atto594-Actin for TIRFM
Non-muscle actin from brain, porcince
Cat. #: 8257-03
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Applications
Fluorescence microscopy, FRET, Fluorescence spectroscopy, Biochemical assays.
Description
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|
Protein |
ATTO594-Actin |
Origin |
Non-muscle β/γ -actin, porcine brain |
Molecular mass |
42kDa |
Protein description |
Actin from non-muscle sources is a mixture of β/γ non-muscle actin isoforms. Both are single chain polypeptides with a molecular weight of 42kD consisting of 375 amino acids. ATTO594-Actin is chemically modified G-actin with an ATTO594 fluorescent group covalently bound to lysine residues. ATTO594-Actin (λex 601nm, λem627nm) possesses the polymerization properties of native G-actin. |
Conjugation ratio |
This product is supplied with a degree of labelling (DOL) ~0.3 and is thus ready.-to-use for TIRFM and other high- or super-resolution imaging techniques. |
Properties
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|
Form |
Lyophilized, ready-to-use. |
Quantity per unit |
2x50 µg |
Buffer |
2.0mM Tris-Cl pH 8.2, 1.0mM ATP, 0.5mM DTT, 0.1mM CaCl2, 1mM NaN3 and 0.3% disaccharides, when reconstituted with 100 µl ultrapure water to obtain a 1mg/ml solution. |
Purity |
>99% by scanning densitometry. |
Purification notes |
Purified by GPC from porcine brain. |
Protein concentration |
Determined by the Biuret method. |
Storage instructions |
ATTO594-Actin stored at –70°C upon arrival will be stable in performance for at least 6 months from the date of purchase. The solubilized product is kept on ice, protect from light and it should be used within 1 week.Avoid refreezing of actin. |
Shipping conditions |
At ambient temperature. Upon delivery store at -70°C. |
Remarks |
For Use in Research only. Not for Use in Human or Veterinary Diagnostical or Therapeutical Applications. |
CAS no. |
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Further Information
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