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ATTO594-Actin (non-muscle actin) - 2x50µg

Cat. #:
8357-01
Shipping:
FedEx Express (Global) FedEx Express (Global) (shipping info)
Quantity:
2x50 µg

 

Atto647-Actin for TIRFM
Non-muscle actin from brain, porcince  

Cat. #: 8258-03

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Applications
Fluorescence microscopy, FRET, Fluorescence spectroscopy, Biochemical assays.

 

Description

 

 

Protein

Atto647-Actin

Origin

Non-muscle β/γ -actin, porcine brain

Molecular mass

42kDa

Protein description

Actin from non-muscle sources is a mixture of β/γ non-muscle actin isoforms. Both are single chain polypeptides with a molecular weight of 42kD consisting of 375 amino acids. Atto647-Actin is chemically modified G-actin with an Atto647 fluorescent group covalently bound to lysine residues. Atto647-Actin (λex 647nm, λem669nm) possesses the polymerization properties of native G-actin. 

Conjugation ratio

This product is supplied with a degree of labelling (DOL) ~0.3 and is thus  ready.-to-use for TIRFM and other high- or super-resolution imaging techniques.



Properties

 

 

Form

Lyophilized, ready-to-use.

Quantity per unit

2x50 µg

Buffer

2.0mM Tris-Cl pH 8.2, 1.0mM ATP, 0.5mM DTT, 0.1mM CaCl2, 1mM NaN3 and 0.3% disaccharides, when reconstituted with 100 µl ultrapure water to obtain a 1mg/ml solution.

Purity

>99% by scanning densitometry.

Purification notes

Purified by GPC from porcine brain.

Protein concentration

Determined by the Biuret method.

Storage instructions

Atto647-Actin stored at –70°C upon arrival will be stable in performance for at least 6 months from the date of purchase. The solubilized product is kept on ice, protect from light and it should be used within 1 week.Avoid refreezing of actin. 

Shipping conditions 

At ambient temperature. Upon delivery store at -70°C.

Remarks

For Use in Research only. Not for Use in Human or Veterinary Diagnostical or Therapeutical Applications. 

CAS no.

 

 



Further Information

Product DataSheet

Material and Safety Data Sheet

Table of Dyes and Light Sources 
 

Protein Sequence on NCBI