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Actin-Toolkit SPR (alpha cardiac muscle actin)

Cat. #:
8900-01
Shipping:
FedEx Express (Global) FedEx Express (Global) (shipping info)
Units:
1x kit
RRP 525,00 EUR
only 420,00 EUR

excl. Shipping costs

Actin-Toolkit SPR
α-actin from bovine cardiac muscle


Cat. #: 8900-01 

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Applications

-   Characterization of binding kinetics and dissociation constants of ligands binding to F-actin;

-   Analysis of proteins, lipids or pharmacological compounds altering ligand-actin-interaction;

 

Description

   

Protein

Actin (α-cardiac muscle Actin)

Origin

Cardiac muscle alpha actin, bovine

Molecular mass

42kDa

Toolkit description

Binding of ligands to filamentous actin is used as a functional proof to state the biological activity of a ligand and characterizes the ligand to be an actin binding protein. Furthermore, the specific actin binding strongly indicates that the ligand is correctly folded and structurally intact.

The Actin-Toolkit SPR provides a unique method to precisely analyze ligand-F-actin interactions by Surface Plasmon Resonance techniques on Streptavidin chips. To overcome the general problem in SPR - which is that the actin filaments clog the capillaries of the device - short actinfilaments with AnkerproteinTM on the fast polymerizing barbed ends are used for for coating. The actin filaments protrude from the surface of the chip and permit the introduction of ligands. After the actin filaments become attached to the surface of the chip, ligands can be introduced to investigate binding to F-actin. 


The Actin-Toolkit SPR was developed to analyze the actin-binding properties of ligands (native or recombinant proteins, recombinant fragments etc.) and to support the detection even of cryptic actin-binding sites. Further it is suitable to investigate effects of pharmacological compounds on ligand-actin complexes, as well as further proteins, lipids etc. to ligand-actin complexes. 

The kit contains
rabbit skeletal muscle actin (Cat. # 8010-03), AnkerproteinTM, FAB-EP, GAP-EP (SPR actin buffers for F-and G-actin). A handbook introduces and guides through the experimental work with actin in SPR.

Please note that the user requires Streptavidin coated chips. The setup for the compounds to investigate has to be determined individually.

Purity

>99% by scanning densitometry.


Properties

   

Form

Lyophilized, ready-to-use.

Kit content

4x250 µg Actin (lyophilized powder);
4x10 µg AnkerproteinTM (lyophilized powder);
1x PolyMix (actin polymerization buffer, 10x);

2x250 ml FAB-EP, liquid (ready-to-use);
1x250 ml GAB-EP, liquid ( ready-to-use);
1xToolkit Handbook SPR;

Buffers

FAB-P: 100mM Imidazol-Cl pH 7.4, 0.4mM ATP, 2mM CaCl2 and 0.0005% v/v Surfactant P20.
GAB-P: 2mM Tris-Cl pH 8.2, 0.4mM ATP, 0.5mM DTT, 0.1mM CaCl2 and 0.0005% v/v Surfactant P20.

Related products

For dilution of G-actin or exchange of ligand buffer into actin compatible buffer, MonoMix (Cat. #: 5100-0*) may be used. In the absence of nucleators, the polymerization of G-actin is initiated by PolyMix (Cat. #: 5000-0*). 

Purification notes

Purified from skeletal muscle, rabbit; GPC.

Protein concentration

Determined by the Biuret method.

Storage instructions

We guarantee stable performance of the kit components for 6 months when stored at -70°C upon arrival. Solubilized G-actin is kept on ice and should be used within 1 week. Avoid refreezing.     

Shipping conditions 

At ambient temperature. Upon delivery store at -70°C.

Remarks

For Use in Research only. Not for Use in Human or Veterinary Diagnostical or Therapeutical Applications.




Further Information
 

 Material and Safety Data Sheet
 

Protein Sequence on NCBI
 



BiacoreTM is a trademark of GE healthcare.
AnkerproteinTM is a trademark of Hypermol.
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