Actin-Trialkit Fluorescence Microscopy ATTO565 (skeletal muscle actin)

Product name

Actin

Target species

Skeletal muscle alpha actin, rabbit

Molecular mass

42kDa

Triallkit description

The Actin-Trialkit Fluorescence Microscopy is used to analyze and demonstrate the effect of ligands on actin polymerization, or ligand-induced formation of actin bundles and networks. In combination with the actin buffers contained in the Trialkit, error-free handling of ATTO565-Actin will be achieved. ATTO565-Actin is a very photostable actin conjugate with λabs=563nm and λfl=592nm and a dye/protein ratio of >0.5, well suited for epifluorescence and laser microscopes.

The kit contains 1x100µg ATTO565-Actin (Cat.# T8031-01), buffers for monomeric & filamentous actin, and the Trialkit Manual. We guarantee stable performance of the kit components for 6 months when stored at -70°C upon arrival. 

Purity

>99% by scanning densitometry.

Form

Lyophilized, ready-to-use.

Kit content

1x100 µg ATTO565-Actin (lyoph. powder);
1x50 ml MonoMix (lyophilized G-actin ATP-buffer);
1x1 ml PolyMix 10x stock (lyophilized F-actin ATP-buffer, pH 7.4);
1x Trialkit Manual Fluorescence Microscopy;

Buffer

2mM Tris-Cl pH 8.2, 0.4mM ATP, 0.5mM DTT, 0.1mM CaCl2, 1mM NaN3 and 0.3% disaccharides, when reconstituted with 1.0 ml ultrapure water to obtain a 1mg/ml solution.

Related products

For dilution of G-actin or exchange of ligand buffer into actin compatible buffer, MonoMix (Cat. #.: 5100-0*) may be used. In the absence of nucleators, the polymerization of G-actin is initiated by PolyMix (Cat.#: 5000-0*). 

Purification notes

Purified from rabbit skeletal muscle, GPC.

Protein concentration

Determined by the Biuret method.

Storage instructions

We guarantee stable performance of the kit components for 6 months when stored at -70°C upon arrival. Solubilized G-actin is kept on ice and should be used within 1 week. Avoid refreezing.     

Shipping conditions 

At ambient temperature. Upon delivery store at -70°C.

Remarks

For Use in Research only. Not for Use in Human or Veterinary Diagnostical or Therapeutical Applications.

CAS no.

51005-14-2 (Actin)