Pyrene Actin 10% (rabbit skeletal muscle alpha actin) - 1.0 mg
TOP
α-actin from rabbit skeletal muscle
Cat. #: 8102-01
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Applications
Fluorimetrical Analysis of actin dynamics (e.g. kinetics of polymerization, nucleation, depolymerization)
Description
Protein |
Pyrene Actin |
Origin |
Skeletal muscle alpha actin, rabbit |
Molecular mass |
42kDa |
Protein description |
G-actin from rabbit skeletal muscle is a single chain polypeptide with a molecular weight of 42kD consisting of 375 amino acids. Pyrene-Actin is chemically modified G-actin, carrying a pyrenyl group covalently bound to Cys374 at the C-term of the molecule. |
Conjugation ratio |
Pyrene Actin (10%) contains 10% of pyrenyl actin with a dye/protein molar ratio of 1. |
Properties
Form |
Lyophilized, ready-to-use. |
Quantity per unit |
1.0 mg |
Buffer |
2mM Tris-Cl pH 8.2, 0.4mM ATP, 0.5mM DTT, 0.1mM CaCl2, 1mM NaN3 and 0.3% disaccharides, when reconstituted with 1.0 ml ultrapure water to obtain a 1mg/ml solution. |
Purity |
>99% by scanning densitometry. |
Purification notes |
Purified by GPC from rabbit skeletal muscle. |
Protein concentration |
Determined by the Biuret method. |
Storage instructions |
Pyrene actin is stored at –70°C upon arrival and will be stable in performance for at least 6 months from the date of purchase. Solubilized pyrene actin is kept on ice and should be used within 1 week. Avoid refreezing. |
Shipping conditions |
At ambient temperature. Upon delivery store at -70°C. |
Remarks |
|
CAS no. |
Further Information
Cysteine-rich protein 2 accelerates actin filament cluster formation.
Kihara T, Sugimoto Y, Shinohara S, Takaoka S, Miyake J.; PLoS One. 2017 Aug 16;12(8):e0183085. doi: 10.1371/journal.pone.0183085. eCollection 2017.
Cooperative effects of tropomyosin on the dynamics of the actin filament.
Khaitlina S, Tsaplina O, Hinssen H.; FEBS Lett. 2017 Jul;591(13):1884-1891. doi: 10.1002/1873-3468.12700. Epub 2017 Jun 11.
Further Information
Cysteine-rich protein 2 accelerates actin filament cluster formation.
Kihara T, Sugimoto Y, Shinohara S, Takaoka S, Miyake J.; PLoS One. 2017 Aug 16;12(8):e0183085. doi: 10.1371/journal.pone.0183085. eCollection 2017.
Cooperative effects of tropomyosin on the dynamics of the actin filament.
Khaitlina S, Tsaplina O, Hinssen H.; FEBS Lett. 2017 Jul;591(13):1884-1891. doi: 10.1002/1873-3468.12700. Epub 2017 Jun 11.
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