Actin-Toolkit SPR (alpha skeletal muscle actin)
Actin-Toolkit SPR
α-actin from skeletal muscle, rabbit
Cat. #: 8090-01
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Applications
- Characterization of binding kinetics and dissociation constants of ligands binding to F-actin;
- Analysis of proteins, lipids or pharmacological compounds altering ligand-actin-interaction;
Description
Protein |
Actin (α-skeletal muscle Actin) |
Origin |
Skeletal muscle alpha actin, rabbit |
Molecular mass |
42kDa |
Toolkit description |
Binding of ligands to filamentous actin is used as a functional proof to state the biological activity of a ligand and characterizes the ligand to be an actin binding protein. Furthermore, the specific actin binding strongly indicates that the ligand is correctly folded and structurally intact. |
Purity |
>99% by scanning densitometry. |
Properties
Form |
Lyophilized, ready-to-use. |
Kit content |
4x250 µg Actin (lyophilized powder); |
Buffers |
FAB-P: 10mM Imidazol-Cl pH 7.4, 0.4mM ATP, 2mM MgCl2 and 0.0005% v/v Surfactant P20. |
Related products |
For dilution of G-actin or exchange of ligand buffer into actin compatible buffer, MonoMix (Cat. #: 5100-0*) may be used. In the absence of nucleators, the polymerization of G-actin is initiated by PolyMix (Cat. #: 5000-0*). |
Purification notes |
Purified from skeletal muscle, rabbit; GPC. |
Protein concentration |
Determined by the Biuret method. |
Storage instructions |
We guarantee stable performance of the kit components for 6 months when stored at -70°C upon arrival. Solubilized G-actin is kept on ice and should be used within 1 week. Avoid refreezing. |
Shipping conditions |
At ambient temperature. Upon delivery store at -70°C. |
Remarks |
For Use in Research only. Not for Use in Human or Veterinary Diagnostical or Therapeutical Applications. |
Further Information
Material and Safety Data Sheet
Material and Safety Data Sheet
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