Rabbit Anti-Guinea Pig IgG ATTO488 (H+L)
Antibody produced in Rabbit, affinity purified
Cat. #: 3703-1MG
Applications
Designed for fluorescence-based methods including immunofluorescence microscopy and flow cytometry. The ATTO488 label is compatible with common 488 nm excitation and FITC/GFP filter sets, supporting multiplex workflows when combined with spectrally distinct fluorophores. Preservative- and protein-additive-free (e.g., no BSA), supporting sensitive staining protocols and live-cell techniques.
Description
| Product name | Rabbit Anti-Guinea Pig IgG (H+L) ATTO488 | RXGP ATTO488 |
| Target species | Guinea Pig |
| Description |
ATTO-labeled secondary antibodies are a new generation of fluorescent reagents characterized by high fluorescence intensity and strong photostability. Rabbit Anti-Guinea Pig IgG (H+L) ATTO488 is optimized for bright green fluorescence detection and is well suited for microscopy and flow cytometry applications requiring robust signal and low photobleaching. The antibody is antigen-specific; affinity purification removes rabbit serum proteins, including immunoglobulins not specifically binding to guinea pig IgG. After dye conjugation, the product is further purified by size-exclusion chromatography (SEC) to remove low-molecular-weight components and support consistent fluorescence performance. This reagent is free of preservatives and protein additives (such as BSA), supporting sensitive fluorescence applications including live-cell techniques. It is supplied as a lyophilized powder (1.0 mg) and shipped at room temperature; a glycerol buffer is provided for convenient reconstitution and subsequent storage at -20°C (including aliquoting for repeated use). |
| Specification | Immunofluorescence: 1:500–1:1500, Degree of Labeling (DOL): 2–9, Unconjugated dye ≤5% of total fluorescence. |
| Optical Properties | λex: 501 nm, λem: 523 nm |
Properties
| Form | Lyophilized |
| Nominal concentration | 2 mg/ml (after reconstitution with buffer) Supplied as 1.0 mg lyophilized antibody; shipped at room temperature. A glycerol buffer is included to support reconstitution and enable storage at -20°C (including aliquoting). |
| Content | 1.0 mg Rabbit Anti-Guinea Pig IgG (H+L) ATTO488 1.0 ml Glycerol buffer (add 0.5 ml to reconstitute the lyophilized antibody) Buffer: 50% glycerol, 0.01 M sodium phosphate, 0.1 M sodium chloride, pH 7.4 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purification notes | Affinity purified. After dye conjugation, the antibody is further purified by size-exclusion chromatography (SEC). |
| Storage instructions | Store as glycerol stock at -20°C. Avoid repeated freeze/thaw cycles. |
| Shipping conditions | Shipped at ambient temperature. |
| Remarks | For Use in Research only. Not for Use in Human or Veterinary Diagnostical or Therapeutical Applications. |
| CAS no. |
Buffers and antibodies from Hypermol® are made of the ultrapure reagents in Milli-Q™ water, as described in our publications.
Further Information
Product DataSheet 
Material and Safety Data Sheet 
Table of Dyes and Light Sources
References
Super-multiplex vibrational imaging.
Wei L, Chen Z, Shi L, Long R, Anzalone AV, Zhang L, Hu F, Yuste R, Cornish VW, Min W.
Nature. 2017 Apr 27;544(7651):465-470. doi: 10.1038/nature22051.
Physiologic and Nanoscale Distinctions Define Glutamatergic Synapses in Tonic vs Phasic Neurons.
He K, Han Y, Li X, et al.
J Neurosci. 2023;43(25):4598-4611. doi:10.1523/JNEUROSCI.0046-23.2023.
Bitbow Enables Highly Efficient Neuronal Lineage Tracing and Morphology Reconstruction in Single Drosophila Brains.
Li Y, et al.
Front Neural Circuits. 2021;15:732183. doi:10.3389/fncir.2021.732183.
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Product DataSheet
Material and Safety Data SheetTable of Dyes and Light Sources
Catalogue: ATTO Secondary Antibodies
Wei L, Chen Z, Shi L, Long R, Anzalone AV, Zhang L, Hu F, Yuste R, Cornish VW, Min W.
Nature. 2017 Apr 27;544(7651):465-470. doi: 10.1038/nature22051. Epub 2017 Apr 19.
αv Integrins combine with LC3 and atg5 to regulate Toll-like receptor signalling in B cells.
Acharya M, Sokolovska A, Tam JM, Conway KL, Stefani C, Raso F, Mukhopadhyay S, Feliu M, Paul E, Savill J, Hynes RO, Xavier RJ, Vyas JM, Stuart LM, Lacy-Hulbert A.
Nat Commun. 2016 Mar 11;7:10917. doi: 10.1038/ncomms10917.
Mechanical signaling coordinates the embryonic heartbeat.
Chiou KK, Rocks JW, Chen CY, Cho S, Merkus KE, Rajaratnam A, Robison P, Tewari M, Vogel K, Majkut SF, Prosser BL, Discher DE, Liu AJ.
Proc Natl Acad Sci U S A. 2016 Aug 9;113(32):8939-44. doi: 10.1073/pnas.1520428113. Epub 2016 Jul 25.
