Rabbit Anti-Human IgG (H+L) ATTO390
Antibody produced in rabbit, affinity purified
Cat. #: 3101-1MG
Applications
Fluorescence-based detection of human IgG in immunoassays and imaging workflows (e.g., immunofluorescence microscopy and multiplex staining). The ATTO390 label supports high-sensitivity readouts and is suited for excitation in the near-UV range; the formulation contains no preservatives.
Description
| Product name | Rabbit Anti-Human IgG (H+L) ATTO390 |
| Target species | Human |
| Description |
ATTO390 is a coumarin-based fluorescent label characterized by high fluorescence quantum yield, a large Stokes shift, and good photostability. Conjugation to ATTO390 provides a bright, photostable signal that supports multiplex workflows with reduced spectral overlap. Rabbit Anti-Human IgG (H+L) ATTO390 is an antigen-specific antibody. Affinity purification removes essentially all rabbit serum proteins, including immunoglobulins not specifically binding human IgG. The antibody is conjugated to ATTO390 and further purified by gel filtration/size-exclusion chromatography. The product is supplied as 1.0 mg lyophilized antibody and shipped at ambient temperature. For convenient reconstitution, a glycerol buffer is provided. This enables freezing at -20°C after use to maintain quality and supports preparation of aliquots for sequential use. The formulation contains no preservatives. |
| Specification | Rabbit Anti-Human IgG (H+L) ATTO390, Immunofluorescence: 1:500–1:1500, Degree of labeling (DOL): 2–9, Unconjugated dye ≤5% of total fluorescence. |
| Optical Properties | λabs: 390 nm, λfl: 476 nm |
Properties
| Form | Lyophilized |
| Nominal concentration | 2 mg/ml (after reconstitution with buffer) Supplied as 1.0 mg lyophilized antibody; shipped at ambient temperature. A glycerol buffer is included to support reconstitution and enable storage at -20°C (including aliquoting). |
| Content | 1.0 mg Rabbit Anti-Human IgG (H+L) ATTO390 1.0 ml Glycerol buffer (add 0.5 ml to reconstitute the lyophilized antibody) Buffer: 50% glycerol, 0.01 M sodium phosphate, 0.1 M sodium chloride, pH 7.4 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purification notes | Column affinity purified. After dye conjugation, the antibody is further purified by gel filtration/size-exclusion chromatography. |
| Storage instructions | Store as glycerol stock at -20°C. Avoid repeated freeze/thaw cycles. |
| Shipping conditions | Shipped at ambient temperature. |
| Remarks | For Use in Research only. Not for Use in Human or Veterinary Diagnostical or Therapeutical Applications. |
Buffers and antibodies from Hypermol® are made of the ultrapure reagents in Milli-Q™ water, as described in our publications.
Further Information
Product DataSheet 
Material and Safety Data Sheet
Table of Dyes and Light Sources
Catalogue: ATTO Secondary AntibodiesReferences
Super-multiplex vibrational imaging.
Wei L, Chen Z, Shi L, Long R, Anzalone AV, Zhang L, Hu F, Yuste R, Cornish VW, Min W.
Nature. 2017 Apr 27;544(7651):465-470. doi: 10.1038/nature22051. Epub 2017 Apr 19.
αv Integrins combine with LC3 and atg5 to regulate Toll-like receptor signalling in B cells.
Acharya M, Sokolovska A, Tam JM, Conway KL, Stefani C, Raso F, Mukhopadhyay S, Feliu M, Paul E, Savill J, Hynes RO, Xavier RJ, Vyas JM, Stuart LM, Lacy-Hulbert A.
Nat Commun. 2016 Mar 11;7:10917. doi: 10.1038/ncomms10917.
Mechanical signaling coordinates the embryonic heartbeat.
Chiou KK, Rocks JW, Chen CY, Cho S, Merkus KE, Rajaratnam A, Robison P, Tewari M, Vogel K, Majkut SF, Prosser BL, Discher DE, Liu AJ.
Proc Natl Acad Sci U S A. 2016 Aug 9;113(32):8939-44. doi: 10.1073/pnas.1520428113. Epub 2016 Jul 25.
Product DataSheet Material and Safety Data SheetTable of Dyes and Light Sources Catalogue: ATTO Secondary Antibodies
Super-multiplex vibrational imaging.
Wei L, Chen Z, Shi L, Long R, Anzalone AV, Zhang L, Hu F, Yuste R, Cornish VW, Min W.
Nature. 2017 Apr 27;544(7651):465-470. doi: 10.1038/nature22051. Epub 2017 Apr 19.
αv Integrins combine with LC3 and atg5 to regulate Toll-like receptor signalling in B cells.
Acharya M, Sokolovska A, Tam JM, Conway KL, Stefani C, Raso F, Mukhopadhyay S, Feliu M, Paul E, Savill J, Hynes RO, Xavier RJ, Vyas JM, Stuart LM, Lacy-Hulbert A.
Nat Commun. 2016 Mar 11;7:10917. doi: 10.1038/ncomms10917.
Mechanical signaling coordinates the embryonic heartbeat.
Chiou KK, Rocks JW, Chen CY, Cho S, Merkus KE, Rajaratnam A, Robison P, Tewari M, Vogel K, Majkut SF, Prosser BL, Discher DE, Liu AJ.
Proc Natl Acad Sci U S A. 2016 Aug 9;113(32):8939-44. doi: 10.1073/pnas.1520428113. Epub 2016 Jul 25.
