Rabbit Anti-Human IgG ATTO490LS (H+L)
Antibody produced in rabbit, affinity purified
Cat. #: 2515-1MG
Applications
Optimized for fluorescence microscopy, flow cytometry, and live-cell imaging. Preservative-free and suitable for high-sensitivity applications.
Description
| Product name | Rabbit Anti-Human IgG ATTO490LS (H+L) | RAH ATTO490LS |
| Target species | Human |
| Description |
ATTO490LS-labeled antibodies are cutting-edge tools for fluorescence-based techniques. Rabbit Anti-Human IgG ATTO490LS (H+L) delivers exceptional brightness, superior photostability, and a large Stokes shift for optimal signal-to-noise ratios in demanding imaging conditions. Rabbit Anti-Human IgG ATTO490LS has a very large stokes shift of 165nm. It is thus specifically suited for multiplexing approaches, especially in combination with ATTO488 and ATTO514 conjugated antibodies. With an excitation/emission spectrum (λex: 496 nm, λem: 661 nm) and a remarkable Stokes shift of 165 nm, this antibody minimizes spectral overlap in multiplex experiments. Its high sensitivity and minimal background fluorescence make it ideal for detecting low-abundance antigens in complex samples. The ATTO490LS dye provides long-lasting fluorescence and compatibility with advanced techniques such as super-resolution microscopy, fluorescence resonance energy transfer (FRET), and spectral unmixing. The antibody is preservative-free to ensure optimal performance in sensitive applications. |
| Specification | Immunofluorescence: 1:500–1:2000, Degree of Labeling (DOL): 4-8, Unconjugated dye ≤5% of total fluorescence. |
| Optical Properties | λex: 496 nm, λem: 661 nm |
Properties
| Form | Lyophilized |
| Nominal concentration | 2 mg/ml (after reconstitution with buffer) The antibody is shipped in unit sizes of 1.0 mg as a lyophilized powder, shipped at room temperature. To reconstitute the antibody before use, a glycerol buffer is included for convenience. This allows to re-freeze the antibody after use at -20°C, to keep the quality and/or to prepare aliquots, which can be stored at -20°C. |
| Content | 1.0 mg Rabbit Anti-Human IgG ATTO490LS (H+L) 1.0 ml Glycerol buffer (add 0.5 ml to reconstitute the lyophilized antibody) Buffer: 50% glycerol, 0.01 M sodium phosphate, 0.1 M sodium chloride, pH 7.4 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purification notes | Affinity purification effectively removed all rabbit serum proteins, including immunoglobulins not specifically binding to human IgG. Following conjugation to ATTO490LS, the antibody was further purified by gel filtration, resulting in a highly pure and specific antibody. |
| Storage instructions | Store as glycerol stock at -20°C. Avoid repeated freeze/thaw cycles. |
| Shipping conditions | Shipped at ambient temperature. |
| Remarks | For Use in Research only. Not for Use in Human or Veterinary Diagnostical or Therapeutical Applications. |
Buffers and antibodies from Hypermol® are made of the purest reagents in Milli-Q™ water, as described in our publications.
Further Information
Material and Safety Data Sheet
References
Super-multiplex vibrational imaging.
Wei L, Chen Z, Shi L, Long R, Anzalone AV, Zhang L, Hu F, Yuste R, Cornish VW, Min W.
Nature. 2017 Apr 27;544(7651):465-470. doi: 10.1038/nature22051.
Physiologic and Nanoscale Distinctions Define Glutamatergic Synapses in Tonic vs Phasic Neurons.
He K, Han Y, Li X, et al.
J Neurosci. 2023;43(25):4598-4611. doi:10.1523/JNEUROSCI.0046-23.2023.
Bitbow Enables Highly Efficient Neuronal Lineage Tracing and Morphology Reconstruction in Single Drosophila Brains.
Li Y, et al.
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Rabbit Anti-Human IgG (H+L) ATTO490LS
Antibody produced in Rabbit, affinity purified
Cat. # 3104-1MG
............................................................................................................................................................................................................................................................................................................................................................................................................
Description
|
Product name |
Rabbit Anti-Human IgG ATTO490LS |
|
Target species |
Human |
|
Description |
ATTO-labeled secondary antibodies belong to the new generation of fluorescent antibodies, characterized by their exceptional fluorescence intensity and superior photostability.
Rabbit Anti-Human IgG ATTO490LS (H+L) is an antigen-specific antibody. Affinity purification removed essentially all Rabbit serum proteins, including immunoglobulins not specifically binding to Human IgG. Rabbit Anti-Human IgG is conjugated to ATTO490LS (Abs. max. 496 nm; Em. max. 661 nm) and further purified by gel filtration. |
|
Specification |
Rabbit Anti-Human IgG ATTO490LS (H+L), Immunofluorescence: 1:500-1:1500, Degree of labeling (DOL) : 2-9, Unconjugated dye ≤5% of total fluorescence. |
|
Optical Properties |
λex 496nm, λem 661nm |
Properties
|
Form |
Lyophilized |
|
Nominal concentration |
2 mg/ml (after reconstitution with buffer) |
|
Content |
1.0 mg Rabbit Anti-Human IgG (H+L) ATTO490LS |
|
Clonality |
Polyclonal |
|
Isotype |
IgG |
|
Purification notes |
Column affinity purified. |
|
Storage instructions |
Store as glycerol stock at -20°C. Avoid repeated freeze / thaw cycles. |
|
Shipping conditions |
Shipped at ambient temperature. |
|
Remarks |
For Use in Research only. Not for Use in Human or Veterinary Diagnostical or Therapeutical Applications. |
|
CAS no. |
Further Information
Product DataSheet 
Material and Safety Data Sheet
Table of Dyes and Light Sources
Catalogue: ATTO Secondary AntibodiesReferences
Super-multiplex vibrational imaging.
Wei L, Chen Z, Shi L, Long R, Anzalone AV, Zhang L, Hu F, Yuste R, Cornish VW, Min W.
Nature. 2017 Apr 27;544(7651):465-470. doi: 10.1038/nature22051. Epub 2017 Apr 19.
αv Integrins combine with LC3 and atg5 to regulate Toll-like receptor signalling in B cells.
Acharya M, Sokolovska A, Tam JM, Conway KL, Stefani C, Raso F, Mukhopadhyay S, Feliu M, Paul E, Savill J, Hynes RO, Xavier RJ, Vyas JM, Stuart LM, Lacy-Hulbert A.
Nat Commun. 2016 Mar 11;7:10917. doi: 10.1038/ncomms10917.
Mechanical signaling coordinates the embryonic heartbeat.
Chiou KK, Rocks JW, Chen CY, Cho S, Merkus KE, Rajaratnam A, Robison P, Tewari M, Vogel K, Majkut SF, Prosser BL, Discher DE, Liu AJ.
Proc Natl Acad Sci U S A. 2016 Aug 9;113(32):8939-44. doi: 10.1073/pnas.1520428113. Epub 2016 Jul 25.
Product DataSheet
Material and Safety Data SheetTable of Dyes and Light Sources Catalogue: ATTO Secondary Antibodies
Bitbow Enables Highly Efficient Neuronal Lineage Tracing and Morphology Reconstruction in Single Drosophila Brains.
Li Y, Walker LA, Zhao Y, Edwards EM, Michki NS, Cheng HPJ, Ghazzi M, Chen TY, Chen M, Roossien DH, Cai D.
Front Neural Circuits. 2021 Oct 20;15:732183.
Super-multiplex vibrational imaging.
Wei L, Chen Z, Shi L, Long R, Anzalone AV, Zhang L, Hu F, Yuste R, Cornish VW, Min W.
Nature. 2017 Apr 27;544(7651):465-470. doi: 10.1038/nature22051. Epub 2017 Apr 19.
αv Integrins combine with LC3 and atg5 to regulate Toll-like receptor signalling in B cells.
Acharya M, Sokolovska A, Tam JM, Conway KL, Stefani C, Raso F, Mukhopadhyay S, Feliu M, Paul E, Savill J, Hynes RO, Xavier RJ, Vyas JM, Stuart LM, Lacy-Hulbert A.
Nat Commun. 2016 Mar 11;7:10917. doi: 10.1038/ncomms10917.
Mechanical signaling coordinates the embryonic heartbeat.
Chiou KK, Rocks JW, Chen CY, Cho S, Merkus KE, Rajaratnam A, Robison P, Tewari M, Vogel K, Majkut SF, Prosser BL, Discher DE, Liu AJ.
Proc Natl Acad Sci U S A. 2016 Aug 9;113(32):8939-44. doi: 10.1073/pnas.1520428113. Epub 2016 Jul 25.
