Rabbit Anti-Human IgG (H+L) ATTO514
Antibody produced in rabbit, affinity purified
Cat. #: 3105-1MG
Applications
Designed for fluorescence-based detection of human IgG in immunoassays, including immunofluorescence microscopy and multiplex staining panels. ATTO514 provides a bright green signal and high photostability, supporting high-resolution imaging and quantitative readouts. Preservative-free formulation supports sensitive workflows, including applications where additives can interfere with downstream steps.
Description
| Product name | Rabbit Anti-Human IgG (H+L) ATTO514 |
| Target species | Human |
| Description |
Rabbit Anti-Human IgG (H+L) ATTO514 is an antigen-specific, affinity-purified antibody for fluorescence-based detection of human IgG. Affinity purification removes essentially all rabbit serum proteins, including immunoglobulins that do not specifically bind human IgG, supporting low background in imaging and assay workflows. The antibody is conjugated to ATTO514 (Abs. max. 511 nm; Em. max. 533 nm) and further purified by gel filtration to remove unconjugated dye and low-molecular-weight components after labeling. The ATTO dye platform is selected for high fluorescence output and photostability, supporting high-resolution microscopy and multiplex staining. The product is supplied preservative-free as a lyophilized powder (1.0 mg) and shipped at ambient temperature. A glycerol buffer is included for convenient reconstitution and subsequent storage at -20°C (including aliquoting to minimize freeze/thaw cycles). |
| Specification | Immunofluorescence: 1:500–1:1500, Degree of labeling (DOL): 2–9, Unconjugated dye ≤5% of total fluorescence. |
| Optical Properties | λex: 511 nm, λem: 533 nm |
Properties
| Form | Lyophilized |
| Nominal concentration | 2 mg/ml (after reconstitution with buffer) Supplied as 1.0 mg lyophilized antibody; shipped at ambient temperature. A glycerol buffer is included to support reconstitution and enable storage at -20°C (including aliquoting). |
| Content | 1.0 mg Rabbit Anti-Human IgG (H+L) ATTO514 1.0 ml Glycerol buffer (add 0.5 ml to reconstitute the lyophilized antibody) Buffer: 50% glycerol, 0.01 M sodium phosphate, 0.1 M sodium chloride, pH 7.4 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purification notes | Column affinity purified. After dye conjugation, the antibody is further purified by gel filtration. |
| Storage instructions | Store as glycerol stock at -20°C. Avoid repeated freeze/thaw cycles. |
| Shipping conditions | Shipped at ambient temperature. |
| Remarks | For Use in Research only. Not for Use in Human or Veterinary Diagnostical or Therapeutical Applications. |
| CAS no. |
Buffers and antibodies from Hypermol® are made of the ultrapure reagents in Milli-Q™ water, as described in our publications.
Further Information
Product DataSheet 
Material and Safety Data Sheet
Table of Dyes and Light Sources
Catalogue: ATTO Secondary AntibodiesReferences
Super-multiplex vibrational imaging.
Wei L, Chen Z, Shi L, Long R, Anzalone AV, Zhang L, Hu F, Yuste R, Cornish VW, Min W.
Nature. 2017 Apr 27;544(7651):465-470. doi: 10.1038/nature22051. Epub 2017 Apr 19.
αv Integrins combine with LC3 and atg5 to regulate Toll-like receptor signalling in B cells.
Acharya M, Sokolovska A, Tam JM, Conway KL, Stefani C, Raso F, Mukhopadhyay S, Feliu M, Paul E, Savill J, Hynes RO, Xavier RJ, Vyas JM, Stuart LM, Lacy-Hulbert A.
Nat Commun. 2016 Mar 11;7:10917. doi: 10.1038/ncomms10917.
Mechanical signaling coordinates the embryonic heartbeat.
Chiou KK, Rocks JW, Chen CY, Cho S, Merkus KE, Rajaratnam A, Robison P, Tewari M, Vogel K, Majkut SF, Prosser BL, Discher DE, Liu AJ.
Proc Natl Acad Sci U S A. 2016 Aug 9;113(32):8939-44. doi: 10.1073/pnas.1520428113. Epub 2016 Jul 25.
Product DataSheet
Material and Safety Data SheetTable of Dyes and Light Sources Catalogue: ATTO Secondary Antibodies
Wei L, Chen Z, Shi L, Long R, Anzalone AV, Zhang L, Hu F, Yuste R, Cornish VW, Min W.
Nature. 2017 Apr 27;544(7651):465-470. doi: 10.1038/nature22051. Epub 2017 Apr 19.
αv Integrins combine with LC3 and atg5 to regulate Toll-like receptor signalling in B cells.
Acharya M, Sokolovska A, Tam JM, Conway KL, Stefani C, Raso F, Mukhopadhyay S, Feliu M, Paul E, Savill J, Hynes RO, Xavier RJ, Vyas JM, Stuart LM, Lacy-Hulbert A.
Nat Commun. 2016 Mar 11;7:10917. doi: 10.1038/ncomms10917.
Mechanical signaling coordinates the embryonic heartbeat.
Chiou KK, Rocks JW, Chen CY, Cho S, Merkus KE, Rajaratnam A, Robison P, Tewari M, Vogel K, Majkut SF, Prosser BL, Discher DE, Liu AJ.
Proc Natl Acad Sci U S A. 2016 Aug 9;113(32):8939-44. doi: 10.1073/pnas.1520428113. Epub 2016 Jul 25.
