Rabbit Anti-Mouse IgG (H+L) ATTO488
Antibody produced in Rabbit, affinity purified
Cat. #: 2403-1MG
Applications
Designed for fluorescence-based detection of mouse primary antibodies in immunofluorescence microscopy, high-resolution imaging, and multiplex staining. The ATTO488 label provides strong fluorescence and high photostability in the green channel, supporting demanding acquisition settings and repeated scanning. Preservative- and protein-additive-free (e.g., no BSA), enabling sensitive workflows and compatibility with live-cell–adjacent handling where required.
Description
| Product name | Rabbit Anti-Mouse IgG (H+L) ATTO488 |
| Target species | Mouse |
| Description |
ATTO-labeled secondary antibodies are a modern class of fluorescent detection reagents characterized by high fluorescence intensity and strong photostability. Rabbit Anti-Mouse IgG (H+L) ATTO488 is a fluorophore-conjugated secondary antibody for sensitive detection of mouse primary antibodies in fluorescence microscopy and related immunoassays. The ATTO488 conjugate is well suited for high-resolution microscopy due to its high photostability and strong fluorescence quantum yield. Rabbit Anti-Mouse IgG (H+L) is antigen-specific and affinity purified to remove essentially all rabbit serum proteins, including immunoglobulins that do not specifically bind mouse IgG. The antibody is conjugated to ATTO488 and further purified by gel filtration to reduce free dye and low-molecular-weight impurities. Optical properties: λex 501 nm, λem 523 nm. The reagent is preservative-free and does not contain protein additives (e.g., no BSA). It is supplied as 1.0 mg lyophilized antibody and shipped at ambient temperature. A glycerol buffer is included for convenient reconstitution and enables storage at -20°C (including aliquoting) for repeated use. |
| Specification | Immunofluorescence: 1:500–1:1500, Degree of labeling (DOL): 2–9, Unconjugated dye ≤5% of total fluorescence. |
| Optical Properties | λex: 501 nm, λem: 523 nm |
Properties
| Form | Lyophilized |
| Nominal concentration | 2 mg/ml (after reconstitution with buffer) |
| Content | 1.0 mg Rabbit Anti-Mouse IgG (H+L) ATTO488 1.0 ml Glycerol buffer (add 0.5 ml to reconstitute the lyophilized antibody) Buffer: 50% glycerol, 0.01 M sodium phosphate, 0.1 M sodium chloride, pH 7.4 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purification notes | Column affinity purified. After dye conjugation, the antibody is further purified by gel filtration. |
| Storage instructions | Store as glycerol stock at -20°C. Avoid repeated freeze/thaw cycles. |
| Shipping conditions | Shipped at ambient temperature. |
| Remarks | For Use in Research only. Not for Use in Human or Veterinary Diagnostical or Therapeutical Applications. |
| CAS no. |
Buffers and antibodies from Hypermol® are made of the ultrapure reagents in Milli-Q™ water, as described in our publications.
Further Information
Product DataSheet 
Material and Safety Data Sheet
Table of Dyes and Light SourcesReferences
Super-multiplex vibrational imaging.
Wei L, Chen Z, Shi L, Long R, Anzalone AV, Zhang L, Hu F, Yuste R, Cornish VW, Min W.
Nature. 2017 Apr 27;544(7651):465-470. doi: 10.1038/nature22051. Epub 2017 Apr 19.
αv Integrins combine with LC3 and atg5 to regulate Toll-like receptor signalling in B cells.
Acharya M, Sokolovska A, Tam JM, Conway KL, Stefani C, Raso F, Mukhopadhyay S, Feliu M, Paul E, Savill J, Hynes RO, Xavier RJ, Vyas JM, Stuart LM, Lacy-Hulbert A.
Nat Commun. 2016 Mar 11;7:10917. doi: 10.1038/ncomms10917.
Mechanical signaling coordinates the embryonic heartbeat.
Chiou KK, Rocks JW, Chen CY, Cho S, Merkus KE, Rajaratnam A, Robison P, Tewari M, Vogel K, Majkut SF, Prosser BL, Discher DE, Liu AJ.
Proc Natl Acad Sci U S A. 2016 Aug 9;113(32):8939-44. doi: 10.1073/pnas.1520428113. Epub 2016 Jul 25.
Product DataSheet
Material and Safety Data SheetTable of Dyes and Light Sources
Catalogue: ATTO Secondary Antibodies
Wei L, Chen Z, Shi L, Long R, Anzalone AV, Zhang L, Hu F, Yuste R, Cornish VW, Min W.
Nature. 2017 Apr 27;544(7651):465-470. doi: 10.1038/nature22051. Epub 2017 Apr 19.
αv Integrins combine with LC3 and atg5 to regulate Toll-like receptor signalling in B cells.
Acharya M, Sokolovska A, Tam JM, Conway KL, Stefani C, Raso F, Mukhopadhyay S, Feliu M, Paul E, Savill J, Hynes RO, Xavier RJ, Vyas JM, Stuart LM, Lacy-Hulbert A.
Nat Commun. 2016 Mar 11;7:10917. doi: 10.1038/ncomms10917.
Mechanical signaling coordinates the embryonic heartbeat.
Chiou KK, Rocks JW, Chen CY, Cho S, Merkus KE, Rajaratnam A, Robison P, Tewari M, Vogel K, Majkut SF, Prosser BL, Discher DE, Liu AJ.
Proc Natl Acad Sci U S A. 2016 Aug 9;113(32):8939-44. doi: 10.1073/pnas.1520428113. Epub 2016 Jul 25.
