Rabbit Anti-Mouse IgG (H+L) ATTO532
Antibody produced in Rabbit, affinity purified
Cat. #: 2406-1MG
Applications
Fluorescent secondary antibody for detection of mouse primary antibodies in immunofluorescence microscopy and related immunoassays. The ATTO532 conjugate is suited for high-resolution imaging workflows where high photostability and strong fluorescence quantum yield support extended acquisition and quantitative readouts. Preservative-free and without protein additives (e.g., no BSA), supporting sensitive staining protocols and compatibility with live-cell–adjacent handling where required.
Description
| Product name | Rabbit Anti-Mouse IgG ATTO532 |
| Target species | Mouse |
| Description |
ATTO-labeled secondary antibodies are fluorescent detection reagents characterized by high fluorescence intensity and strong photostability. Rabbit Anti-Mouse IgG (H+L) ATTO532 is intended for sensitive detection of mouse primary antibodies in fluorescence microscopy and related immunoassays, including high-resolution imaging applications. The antibody is preservative-free and does not contain protein additives (e.g., no BSA). It is suitable for a broad range of immunobased applications. Rabbit Anti-Mouse IgG ATTO532 (H+L) is antigen-specific and affinity purified to remove essentially all rabbit serum proteins, including immunoglobulins that do not specifically bind mouse IgG. The antibody is conjugated to ATTO532 (Abs. max. 532 nm; Em. max. 553 nm) and further purified by gel filtration. The product is supplied as 1.0 mg lyophilized antibody and shipped at ambient temperature. A glycerol buffer is included for convenient reconstitution and enables storage at -20°C (including aliquoting) for repeated use. |
| Specification | Immunofluorescence: 1:500–1:1500, Degree of labeling (DOL): 2–9, Unconjugated dye ≤5% of total fluorescence. |
| Optical Properties | λex: 532 nm, λem: 553 nm |
Properties
| Form | Lyophilized |
| Nominal concentration | 2 mg/ml (after reconstitution with buffer) |
| Content | 1.0 mg Rabbit Anti-Mouse IgG (H+L) ATTO532 1.0 ml Glycerol buffer (add 0.5 ml to reconstitute the lyophilized antibody) Buffer: 50% glycerol, 0.01 M sodium phosphate, 0.1 M sodium chloride, pH 7.4 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purification notes | Column affinity purified. After dye conjugation, the antibody is further purified by gel filtration. |
| Storage instructions | Store as glycerol stock at -20°C. Avoid repeated freeze/thaw cycles. |
| Shipping conditions | Shipped at ambient temperature. |
| Remarks | For Use in Research only. Not for Use in Human or Veterinary Diagnostical or Therapeutical Applications. |
| CAS no. |
Buffers and antibodies from Hypermol® are made of the ultrapure reagents in Milli-Q™ water, as described in our publications.
Further Information
Product DataSheet 
Material and Safety Data Sheet
Table of Dyes and Light SourcesReferences
Super-multiplex vibrational imaging.
Wei L, Chen Z, Shi L, Long R, Anzalone AV, Zhang L, Hu F, Yuste R, Cornish VW, Min W.
Nature. 2017 Apr 27;544(7651):465-470. doi: 10.1038/nature22051. Epub 2017 Apr 19.
αv Integrins combine with LC3 and atg5 to regulate Toll-like receptor signalling in B cells.
Acharya M, Sokolovska A, Tam JM, Conway KL, Stefani C, Raso F, Mukhopadhyay S, Feliu M, Paul E, Savill J, Hynes RO, Xavier RJ, Vyas JM, Stuart LM, Lacy-Hulbert A.
Nat Commun. 2016 Mar 11;7:10917. doi: 10.1038/ncomms10917.
Mechanical signaling coordinates the embryonic heartbeat.
Chiou KK, Rocks JW, Chen CY, Cho S, Merkus KE, Rajaratnam A, Robison P, Tewari M, Vogel K, Majkut SF, Prosser BL, Discher DE, Liu AJ.
Proc Natl Acad Sci U S A. 2016 Aug 9;113(32):8939-44. doi: 10.1073/pnas.1520428113. Epub 2016 Jul 25.
Product DataSheet
Material and Safety Data SheetTable of Dyes and Light Sources
Catalogue: ATTO Secondary Antibodies
Wei L, Chen Z, Shi L, Long R, Anzalone AV, Zhang L, Hu F, Yuste R, Cornish VW, Min W.
Nature. 2017 Apr 27;544(7651):465-470. doi: 10.1038/nature22051. Epub 2017 Apr 19.
αv Integrins combine with LC3 and atg5 to regulate Toll-like receptor signalling in B cells.
Acharya M, Sokolovska A, Tam JM, Conway KL, Stefani C, Raso F, Mukhopadhyay S, Feliu M, Paul E, Savill J, Hynes RO, Xavier RJ, Vyas JM, Stuart LM, Lacy-Hulbert A.
Nat Commun. 2016 Mar 11;7:10917. doi: 10.1038/ncomms10917.
Mechanical signaling coordinates the embryonic heartbeat.
Chiou KK, Rocks JW, Chen CY, Cho S, Merkus KE, Rajaratnam A, Robison P, Tewari M, Vogel K, Majkut SF, Prosser BL, Discher DE, Liu AJ.
Proc Natl Acad Sci U S A. 2016 Aug 9;113(32):8939-44. doi: 10.1073/pnas.1520428113. Epub 2016 Jul 25.
