Product name

Rabbit Anti-Mouse IgG ATTO647N

Target species

Mouse

Description

Rabbit Anti-Mouse IgG ATTO647N is specifically suited for high resolution microscopy, due to the high photostability and strong fluorescence quantum yield. 

It is thus specifically suited for all multiplex approaches. The antibody is used for all immunobased applications, including live cell techniques, since it contains no preservatives.

Rabbit Anti-Mouse IgG ATTO647N (H+L) is an antigen-specific antibody. Affinity purification removed essentially all Rabbit serum proteins, including immunoglobulins not specifically binding to Mouse IgG. Rabbit Anti-Mouse IgG is conjugated to ATTO647N (Abs.max. 647 nm; Em.max. 669 nm) and further purified by gel filtration.
 
Rabbit Anti-Mouse IgG ATTO647N (H+L) is supplied as a 1.0 mg lyophilized powder and is shipped at room temperature. For convenient reconstitution, a glycerol buffer is provided. This enables re-freezing of the antibody at -20°C after use to maintain quality and allows for the preparation of aliquots, which can also be stored at -20°C for sequential use.

Specification

Rabbit Anti-Mouse IgG ATTO647N (H+L), Immunofluorescence: 1:500-1:1500, Degree of labeling (DOL) : 2-9, Unconjugated dye ≤5% of total fluorescence. 

Optical Properties

λex 647m, λem 669nm

Form

Lyophilized

Nominal concentration

2 mg/ml (after reconstitution with buffer)

Content



Buffer

1.0 mg Rabbit Anti-Mouse IgG (H+L) ATTO647N
1.0 ml Glycerol buffer (add 0.5ml to reconstitute the lyophilized antibody)


50% glycerol, 0.01 M sodium phosphate, 0.1 M sodium chloride, pH 7.4.

Clonality

Polyclonal

Isotype

IgG

Purification notes

Column affinity purified.

Storage instructions

Store as glycerol stock at -20°C. Avoid repeated freeze / thaw cycles.

Shipping conditions 

Shipped at ambient temperature.

Remarks

For Use in Research only. Not for Use in Human or Veterinary Diagnostical or Therapeutical Applications. 

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