Rabbit Anti-Sheep IgG ATTO490LS (H+L)
Antibody produced in rabbit, affinity purified
Cat. #: 3304-1MG
Applications
Designed for high-performance fluorescence microscopy, flow cytometry, and live-cell imaging. Preservative-free to support high-sensitivity applications in research and diagnostics.
Description
| Product name | Rabbit Anti-Sheep IgG ATTO490LS (H+L) | RXSh ATTO490LS |
| Target species | Sheep |
| Description |
ATTO490LS-labeled antibodies are advanced tools for fluorescence-based applications. Rabbit Anti-Sheep IgG ATTO490LS (H+L) is distinguished by its exceptional fluorescence intensity, superior photostability, and extended Stokes shift, making it an excellent choice for demanding experimental conditions. This antibody has an excitation/emission spectrum (λex: 490 nm, λem: 655 nm) and a substantial Stokes shift of 165 nm. These properties provide optimal spectral separation for multiplex imaging and enhanced detection of low-abundance targets with minimal background interference. ATTO490LS offers high compatibility with advanced techniques such as super-resolution microscopy, fluorescence resonance energy transfer (FRET), and spectral unmixing. The antibody’s preservative-free formulation ensures high compatibility with sensitive experiments, providing reliable performance and reproducibility. |
| Specification | Immunofluorescence: 1:500–1:2000, Degree of Labeling (DOL): 4-8, Unconjugated dye ≤5% of total fluorescence. |
| Optical Properties | λex: 490 nm, λem: 655 nm |
Properties
| Form | Lyophilized |
| Nominal concentration | 2 mg/ml (after reconstitution with buffer) The antibody is shipped in unit sizes of 1.0 mg as a lyophilized powder, shipped at room temperature. To reconstitute the antibody before use, a glycerol buffer is included for convenience. This allows to re-freeze the antibody after use at -20°C, to keep the quality and/or to prepare aliquots, which can be stored at -20°C. |
| Content | 1.0 mg Rabbit Anti-Sheep IgG ATTO490LS (H+L) 1.0 ml Glycerol buffer (add 0.5 ml to reconstitute the lyophilized antibody) Buffer: 50% glycerol, 0.01 M sodium phosphate, 0.1 M sodium chloride, pH 7.4 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purification notes | Affinity purification effectively removed all rabbit serum proteins, including immunoglobulins not specifically binding to sheep IgG. Following conjugation to ATTO490LS, the antibody was further purified by gel filtration, resulting in a highly pure and specific antibody. |
| Storage instructions | Store as glycerol stock at -20°C. Avoid repeated freeze/thaw cycles. |
| Shipping conditions | Shipped at ambient temperature. |
| Remarks | For Use in Research only. Not for Use in Human or Veterinary Diagnostical or Therapeutical Applications. |
Buffers and antibodies from Hypermol® are made of the purest reagents in Milli-Q™ water, as described in our publications.
Further Information
Material and Safety Data Sheet
References
Super-multiplex vibrational imaging.
Wei L, Chen Z, Shi L, Long R, Anzalone AV, Zhang L, Hu F, Yuste R, Cornish VW, Min W.
Nature. 2017 Apr 27;544(7651):465-470. doi: 10.1038/nature22051.
Physiologic and Nanoscale Distinctions Define Glutamatergic Synapses in Tonic vs Phasic Neurons.
He K, Han Y, Li X, et al.
J Neurosci. 2023;43(25):4598-4611. doi:10.1523/JNEUROSCI.0046-23.2023.
Bitbow Enables Highly Efficient Neuronal Lineage Tracing and Morphology Reconstruction in Single Drosophila Brains.
Li Y, et al.
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Product DataSheet
Material and Safety Data Sheet
Table of Dyes and Light Sources
Catalogue: ATTO Secondary Antibodies
Bitbow Enables Highly Efficient Neuronal Lineage Tracing and Morphology Reconstruction in Single Drosophila Brains.
Li Y, Walker LA, Zhao Y, Edwards EM, Michki NS, Cheng HPJ, Ghazzi M, Chen TY, Chen M, Roossien DH, Cai D.
Front Neural Circuits. 2021 Oct 20;15:732183.
Super-multiplex vibrational imaging.
Wei L, Chen Z, Shi L, Long R, Anzalone AV, Zhang L, Hu F, Yuste R, Cornish VW, Min W.
Nature. 2017 Apr 27;544(7651):465-470. doi: 10.1038/nature22051. Epub 2017 Apr 19.
αv Integrins combine with LC3 and atg5 to regulate Toll-like receptor signalling in B cells.
Acharya M, Sokolovska A, Tam JM, Conway KL, Stefani C, Raso F, Mukhopadhyay S, Feliu M, Paul E, Savill J, Hynes RO, Xavier RJ, Vyas JM, Stuart LM, Lacy-Hulbert A.
Nat Commun. 2016 Mar 11;7:10917. doi: 10.1038/ncomms10917.
Mechanical signaling coordinates the embryonic heartbeat.
Chiou KK, Rocks JW, Chen CY, Cho S, Merkus KE, Rajaratnam A, Robison P, Tewari M, Vogel K, Majkut SF, Prosser BL, Discher DE, Liu AJ.
Proc Natl Acad Sci U S A. 2016 Aug 9;113(32):8939-44. doi: 10.1073/pnas.1520428113. Epub 2016 Jul 25.
