Rabbit Anti-Sheep IgG (H+L) ATTO490LS
Antibody produced in rabbit, affinity purified
Cat. #: 3304-1MG
Applications
Designed for fluorescence-based workflows including immunofluorescence microscopy, multiplex imaging, and spectral unmixing. The large Stokes shift of ATTO490LS supports robust channel separation and reduced bleed-through in multi-color experiments. Preservative- and protein-additive-free (e.g., no BSA), supporting sensitive applications and low-background staining.
Description
| Product name | Rabbit Anti-Sheep IgG (H+L) ATTO490LS |
| Target species | Sheep |
| Description |
ATTO-labeled secondary antibodies are high-performance fluorescent reagents characterized by high signal intensity and strong photostability. Rabbit Anti-Sheep IgG (H+L) ATTO490LS is antigen-specific and affinity purified to remove essentially all rabbit serum proteins, including immunoglobulins not specifically binding to sheep IgG. The antibody is conjugated to ATTO490LS (Abs. max. 490 nm | Em. max. 655 nm) and provides a large Stokes shift of 165 nm, supporting multiplex imaging and spectral separation with reduced background interference. Following conjugation, the product is further purified by gel filtration/size-exclusion to minimize unbound dye and low-molecular-weight impurities. This reagent is free of preservatives and protein additives (such as BSA), supporting sensitive fluorescence applications. It is supplied as a lyophilized powder (1.0 mg) and shipped at ambient temperature; a glycerol buffer is provided for convenient reconstitution and subsequent storage at -20°C (including aliquoting for repeated use). |
| Specification | Immunofluorescence: 1:500–1:2000, Degree of labeling (DOL): 4–8, Unconjugated dye ≤5% of total fluorescence. |
| Optical Properties | Abs. max.: 490 nm, Em. max.: 655 nm |
Properties
| Form | Lyophilized |
| Nominal concentration | 2 mg/ml (after reconstitution with buffer) Supplied as 1.0 mg lyophilized antibody; shipped at ambient temperature. A glycerol buffer is included to support reconstitution and enable storage at -20°C (including aliquoting). |
| Content | 1.0 mg Rabbit Anti-Sheep IgG (H+L) ATTO490LS 1.0 ml Glycerol buffer (add 0.5 ml to reconstitute the lyophilized antibody) Buffer: 50% glycerol, 0.01 M sodium phosphate, 0.1 M sodium chloride, pH 7.4 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purification notes | Affinity purified. After dye conjugation, the antibody is further purified by gel filtration/size-exclusion to reduce unbound dye and support low background. |
| Storage instructions | Store as glycerol stock at -20°C. Avoid repeated freeze/thaw cycles. |
| Shipping conditions | Shipped at ambient temperature. |
| Remarks | For Use in Research only. Not for Use in Human or Veterinary Diagnostical or Therapeutical Applications. |
Buffers and antibodies from Hypermol® are made of the ultrapure reagents in Milli-Q™ water, as described in our publications.
Further Information
Material and Safety Data Sheet
References
Super-multiplex vibrational imaging.
Wei L, Chen Z, Shi L, Long R, Anzalone AV, Zhang L, Hu F, Yuste R, Cornish VW, Min W.
Nature. 2017 Apr 27;544(7651):465-470. doi: 10.1038/nature22051.
Physiologic and Nanoscale Distinctions Define Glutamatergic Synapses in Tonic vs Phasic Neurons.
He K, Han Y, Li X, et al.
J Neurosci. 2023;43(25):4598-4611. doi:10.1523/JNEUROSCI.0046-23.2023.
Bitbow Enables Highly Efficient Neuronal Lineage Tracing and Morphology Reconstruction in Single Drosophila Brains.
Li Y, et al.
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Product DataSheet
Material and Safety Data Sheet
Table of Dyes and Light Sources
Catalogue: ATTO Secondary Antibodies
Bitbow Enables Highly Efficient Neuronal Lineage Tracing and Morphology Reconstruction in Single Drosophila Brains.
Li Y, Walker LA, Zhao Y, Edwards EM, Michki NS, Cheng HPJ, Ghazzi M, Chen TY, Chen M, Roossien DH, Cai D.
Front Neural Circuits. 2021 Oct 20;15:732183.
Super-multiplex vibrational imaging.
Wei L, Chen Z, Shi L, Long R, Anzalone AV, Zhang L, Hu F, Yuste R, Cornish VW, Min W.
Nature. 2017 Apr 27;544(7651):465-470. doi: 10.1038/nature22051. Epub 2017 Apr 19.
αv Integrins combine with LC3 and atg5 to regulate Toll-like receptor signalling in B cells.
Acharya M, Sokolovska A, Tam JM, Conway KL, Stefani C, Raso F, Mukhopadhyay S, Feliu M, Paul E, Savill J, Hynes RO, Xavier RJ, Vyas JM, Stuart LM, Lacy-Hulbert A.
Nat Commun. 2016 Mar 11;7:10917. doi: 10.1038/ncomms10917.
Mechanical signaling coordinates the embryonic heartbeat.
Chiou KK, Rocks JW, Chen CY, Cho S, Merkus KE, Rajaratnam A, Robison P, Tewari M, Vogel K, Majkut SF, Prosser BL, Discher DE, Liu AJ.
Proc Natl Acad Sci U S A. 2016 Aug 9;113(32):8939-44. doi: 10.1073/pnas.1520428113. Epub 2016 Jul 25.
