Product name

Rabbit Anti-Sheep IgG ATTO594 (H+L)

Target species

Sheep

Description

ATTO-labelled secondary antibodies belong to the new generation of fluorescent antibodies characterized by their exceptional fluorescence intensity and superior photostability. 

Rabbit Anti-Sheep ATTO594 is specifically suited for high resolution microscopy, due to the high photostability and strong fluorescence quantum yield. The antibody is suitable for a wide range of applications, including live cell techniques, as it is free from preservatives and protein additives such as BSA.


Rabbit Anti-Sheep IgG ATTO594 (H+L) is an antigen-specific antibody. Affinity purification removed essentially all rabbit serum proteins, including immunoglobulins not specifically binding to Sheep IgG. Rabbit Anti-Sheep IgG is conjugated to ATTO594 (Abs.max. 601 nm; Em.max. 627 nm) and further purified by SEC.
 
Rabbit Anti-Sheep ATTO594 is supplied as a 1.0 mg lyophilized powder and is shipped at room temperature. For convenient reconstitution, a glycerol buffer is provided. This enables re-freezing of the antibody at -20°C after use to maintain quality and allows for the preparation of aliquots, which can also be stored at -20°C for sequential use.

Specification

Rabbit Anti-Sheep IgG ATTO594 (H+L), Immunofluorescence: 1:500-1:1500, Degree of labelling (DOL) : 2-9, Unconjugated dye ≤5% of total fluorescence. 

Optical Properties

λex 601 nm, λem 627 nm

Form

Lyophilized

Nominal concentration

2 mg/ml (after reconstitution with buffer)

Content



Buffer

1.0 mg Rabbit Anti-Sheep IgG ATTO594 (H+L)
1.0 ml Glycerol buffer (add 0.5ml to reconstitute the lyophilized antibody)


50% glycerol, 0.01 M sodium phosphate, 0.1 M sodium chloride, pH 7.4.

Clonality

Polyclonal

Isotype

IgG

Purification notes

Affinity purification effectively removed all rabbit serum proteins, including immunoglobulins not specifically binding to sheep IgG. After conjugation to the dye the antibody was further purified by gel filtration, resulting in a highly pure and specific antibody.

Hypermol® secondary antibodies are highly specific and purified to remove unbound dye, minimizing background signal.

Storage instructions

Store as glycerol stock at -20°C. Avoid repeated freeze / thaw cycles.

Shipping conditions 

Shipped at ambient temperature.

Remarks

 For Use in Research only. Not for Use in Sheep or Veterinary Diagnostical or Therapeutical Applications. 

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