Rabbit Anti-Sheep IgG (H+L) ATTO647N
Antibody produced in rabbit, affinity purified
Cat. #: 3321-1MG
Applications
Designed for fluorescence-based detection in immunofluorescence microscopy and multiplex staining. The ATTO647N fluorophore provides high photostability and strong fluorescence quantum yield, supporting high-resolution imaging workflows. Preservative- and protein-additive-free (e.g., no BSA) to help minimize background and improve compatibility with sensitive experiments, including live-cell techniques.
Description
| Product name | Rabbit Anti-Sheep IgG (H+L) ATTO647N |
| Target species | Sheep |
| Description |
Rabbit Anti-Sheep IgG (H+L) ATTO647N is an antigen-specific secondary antibody for fluorescence applications. Affinity purification removed essentially all rabbit serum proteins, including immunoglobulins not specifically binding to sheep IgG. The antibody is conjugated to ATTO647N (Abs. max. 647 nm | Em. max. 669 nm) and further purified by size-exclusion chromatography (SEC) to reduce unbound dye and support low background. The formulation is preservative-free and free of protein additives such as BSA, supporting sensitive workflows including live-cell techniques. The antibody is supplied as 1.0 mg lyophilized powder and shipped at ambient temperature. A glycerol buffer is included for convenient reconstitution and storage at -20°C (including aliquoting for repeated use). |
| Specification | Immunofluorescence: 1:500–1:1500, Degree of labeling (DOL): 2–9, Unconjugated dye ≤5% of total fluorescence. |
| Optical Properties | Abs. max.: 647 nm, Em. max.: 669 nm |
Properties
| Form | Lyophilized |
| Nominal concentration | 2 mg/ml (after reconstitution with buffer) |
| Content | 1.0 mg Rabbit Anti-Sheep IgG (H+L) ATTO647N 1.0 ml Glycerol buffer (add 0.5 ml to reconstitute the lyophilized antibody) Buffer: 50% glycerol, 0.01 M sodium phosphate, 0.1 M sodium chloride, pH 7.4 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purification notes | Affinity purification effectively removed all rabbit serum proteins, including immunoglobulins not specifically binding to sheep IgG. After dye conjugation, the antibody is further purified by size-exclusion chromatography (SEC) to reduce unbound dye and support low background. |
| Storage instructions | Store as glycerol stock at -20°C. Avoid repeated freeze/thaw cycles. |
| Shipping conditions | Shipped at ambient temperature. |
| Remarks | For Use in Research only. Not for Use in Sheep or Veterinary Diagnostical or Therapeutical Applications. |
Buffers and antibodies from Hypermol® are made of the ultrapure reagents in Milli-Q™ water, as described in our publications.
Further Information
Product DataSheet 
Material and Safety Data Sheet
Table of Dyes and Light SourcesReferences
Super-multiplex vibrational imaging.
Wei L, Chen Z, Shi L, Long R, Anzalone AV, Zhang L, Hu F, Yuste R, Cornish VW, Min W.
Nature. 2017 Apr 27;544(7651):465-470. doi: 10.1038/nature22051. Epub 2017 Apr 19.
αv Integrins combine with LC3 and atg5 to regulate Toll-like receptor signalling in B cells.
Acharya M, Sokolovska A, Tam JM, Conway KL, Stefani C, Raso F, Mukhopadhyay S, Feliu M, Paul E, Savill J, Hynes RO, Xavier RJ, Vyas JM, Stuart LM, Lacy-Hulbert A.
Nat Commun. 2016 Mar 11;7:10917. doi: 10.1038/ncomms10917.
Mechanical signaling coordinates the embryonic heartbeat.
Chiou KK, Rocks JW, Chen CY, Cho S, Merkus KE, Rajaratnam A, Robison P, Tewari M, Vogel K, Majkut SF, Prosser BL, Discher DE, Liu AJ.
Proc Natl Acad Sci U S A. 2016 Aug 9;113(32):8939-44. doi: 10.1073/pnas.1520428113. Epub 2016 Jul 25.
Product DataSheet
Material and Safety Data SheetTable of Dyes and Light Sources
Catalogue: ATTO Secondary Antibodies
Wei L, Chen Z, Shi L, Long R, Anzalone AV, Zhang L, Hu F, Yuste R, Cornish VW, Min W.
Nature. 2017 Apr 27;544(7651):465-470. doi: 10.1038/nature22051. Epub 2017 Apr 19.
αv Integrins combine with LC3 and atg5 to regulate Toll-like receptor signalling in B cells.
Acharya M, Sokolovska A, Tam JM, Conway KL, Stefani C, Raso F, Mukhopadhyay S, Feliu M, Paul E, Savill J, Hynes RO, Xavier RJ, Vyas JM, Stuart LM, Lacy-Hulbert A.
Nat Commun. 2016 Mar 11;7:10917. doi: 10.1038/ncomms10917.
Mechanical signaling coordinates the embryonic heartbeat.
Chiou KK, Rocks JW, Chen CY, Cho S, Merkus KE, Rajaratnam A, Robison P, Tewari M, Vogel K, Majkut SF, Prosser BL, Discher DE, Liu AJ.
Proc Natl Acad Sci U S A. 2016 Aug 9;113(32):8939-44. doi: 10.1073/pnas.1520428113. Epub 2016 Jul 25.
