Actin-Toolkit Fluorescence Microscopy
Bovine α-cardiac Rhodamine-Actin
Cat. # 8800-01
......................................................................................................................................................................................................
Applications
- Visualization of ligand-induced actin polymerization;
- Microscopical analysis of actin filament bundles or networks;
Description
The Actin-Toolkit Fluorescence Microscopy was developed to analyze and demonstrate the effect of ligands on actin polymerization, or ligand-induced formation of actin bundles and networks. In the assays Rhodamine-Actin is mixed with the ligand to observe the effects on either G- or F-actin. In contrast to staining with phalloidin conjugates, which can interfere with ligand binding sites on the actin molecule, no such observations were made with rhodamine labeled actin.
Due to the superior features of the Atto-dyes we recommend using the Fluorescence Toolkits with Atto532- or Atto565-Actin.
The Toolkit further contains unlabeled actin (0.5mg α-cardiac actin). A mixture of both excludes possible interference of the fluorescent actin conjugates on ligand binding sites.
Several standardized experiments are described step-by-step in the handbook supplied with the kit. In combination with the actinbuffers supplied with the kit, error-free handling is guaranteed. The kit contains 4x100µg of α-cardiac Rhodamine-Actin conjugates and buffers to keep the actin functional.
We guarantee excellent performance of kit components when stored at -70°C upon arrival for 6 months.
Kit content (50-100 assays)
4x100µg Rhodamine-Actin (lyophilized powder);
1x0.5mg α-cardiac Actin (lyophilized powder);
5x50ml MonoMix (lyophilized G-actin ATP-buffer);
1x1.0ml PolyMix 10x stock (lyophilized F-actin ATP-buffer, pH 7.4);
1x0.5ml 100mM MgCl2;
1x Toolkit Handbook Fluorescence Microscopy;
10x Light protective tubes & tags;
Further Information
Material and Safety Data Sheet
Table of Dyes and Light Sources
Protein Sequence on NCBI
Handbook Actin-Toolkit Fluorescence Microscopy
(Web version only for demonstration purpose!)
A full print version with protocols come with the kit.
Flyer Actin-Toolkits
Material and Safety Data Sheet
Protein Sequence on NCBI