Sheep Anti-Mouse IgG ATTO700 (H+L)
Antibody produced in sheep, affinity purified
Cat. #: 3511-1MG
Applications
Suited for microscopy and live-cell techniques, containing no preservatives for compatibility with sensitive applications.
Description
| Product name | Sheep Anti-Mouse IgG ATTO700 (H+L) | SXM ATTO700 |
| Target species | Mouse |
| Description |
ATTO-labeled secondary antibodies provide bright and photostable fluorescence for sensitive detection of primary antibodies in fluorescence-based immunoassays. Sheep Anti-Mouse IgG ATTO700 (H+L) is affinity purified and designed for far-red detection of mouse primary antibodies, recognizing both heavy and light chains. ATTO700 exhibits excitation/emission maxima of λex: 700 nm and λem: 716 nm. The long-wavelength emission supports far-red imaging workflows and can help reduce sample autofluorescence compared with shorter-wavelength channels, improving signal-to-background in complex specimens. This antibody is preservative-free to ensure compatibility with sensitive applications, including live-cell and extended imaging workflows where preservative components may be undesirable. |
| Specification | Immunofluorescence: 1:500–1:1500, Degree of Labeling (DOL): 2-5, Unconjugated dye ≤5% of total fluorescence. |
| Optical Properties | λex: 700 nm, λem: 716 nm |
Properties
| Form | Lyophilized |
| Nominal concentration | 2 mg/ml (after reconstitution with buffer) |
| Content | 1.0 mg Sheep Anti-Mouse IgG ATTO700 (H+L) 1.0 ml Glycerol buffer (add 0.5 ml to reconstitute the lyophilized antibody) Buffer: 50% glycerol, 0.01 M sodium phosphate, 0.1 M sodium chloride, pH 7.4 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purification notes | Affinity purification effectively removed all sheep serum proteins, including immunoglobulins not specifically binding to mouse IgG. |
| Storage instructions | Store as glycerol stock at -20°C. Avoid repeated freeze/thaw cycles. |
| Shipping conditions | Shipped at ambient temperature. |
| Remarks | For Use in Research only. Not for Use in Human or Veterinary Diagnostical or Therapeutical Applications. |
Buffers and antibodies from Hypermol® are made of the ultrapure reagents in Milli-Q™ water, as described in our publications.
Further Information
Product DataSheet
Material and Safety Data Sheet
References
Super-multiplex vibrational imaging.
Wei L, Chen Z, Shi L, Long R, Anzalone AV, Zhang L, Hu F, Yuste R, Cornish VW, Min W.
Nature. 2017 Apr 27;544(7651):465-470. doi: 10.1038/nature22051.
Physiologic and Nanoscale Distinctions Define Glutamatergic Synapses in Tonic vs Phasic Neurons.
He K, Han Y, Li X, et al.
J Neurosci. 2023;43(25):4598-4611. doi:10.1523/JNEUROSCI.0046-23.2023.
Bitbow Enables Highly Efficient Neuronal Lineage Tracing and Morphology Reconstruction in Single Drosophila Brains.
Li Y, et al.
Front Neural Circuits. 2021;15:732183. doi:10.3389/fncir.2021.732183.
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