Sheep Anti-Mouse IgG ATTO514 (H+L)
Antibody produced in sheep, affinity purified
Cat. #: 3505-1MG
Applications
Suited for microscopy and live-cell techniques, containing no preservatives for compatibility with sensitive applications.
Description
| Product name | Sheep Anti-Mouse IgG ATTO514 (H+L) | SXM ATTO514 |
| Target species | Mouse |
| Description |
ATTO-labeled secondary antibodies represent a new generation of fluorescent reagents, characterized by their exceptional fluorescence intensity, superior photostability, and consistent performance across various applications. Sheep Anti-Mouse IgG ATTO514 (H+L) is optimized for high-resolution fluorescence microscopy, ensuring brilliant and stable signals for prolonged imaging. ATTO514 exhibits a distinct excitation/emission spectrum (λex: 511 nm, λem: 532 nm), offering excellent compatibility with standard fluorescence microscopy systems. Its high quantum yield and minimal autofluorescence make it ideal for detecting low-abundance targets and achieving precise spatial resolution, even in challenging biological samples. This antibody is preservative-free, making it suitable for live-cell imaging and other sensitive applications. Its low non-specific binding and high affinity ensure accurate target detection, while its robust fluorescence properties allow consistent results in advanced techniques such as flow cytometry, super-resolution microscopy, and fluorescence resonance energy transfer (FRET) assays. |
| Specification | Immunofluorescence: 1:500–1:1500, Degree of Labeling (DOL): 2-5, Unconjugated dye ≤5% of total fluorescence. |
| Optical Properties | λex: 511 nm, λem: 532 nm |
Properties
| Form | Lyophilized |
| Nominal concentration | 2 mg/ml (after reconstitution with buffer) The antibody is shipped in unit sizes of 1.0 mg as a lyophilized powder, shipped at room temperature. To reconstitute the antibody before use, a glycerol buffer is included for convenience. This allows to re-freeze the antibody after use at -20°C, to keep the quality and/or to prepare aliquots, which can be stored at -20°C. |
| Content | 1.0 mg Sheep Anti-Mouse IgG ATTO514 (H+L) 1.0 ml Glycerol buffer (add 0.5 ml to reconstitute the lyophilized antibody) Buffer: 50% glycerol, 0.01 M sodium phosphate, 0.1 M sodium chloride, pH 7.4 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purification notes | Affinity purification effectively removed all sheep serum proteins, including immunoglobulins not specifically binding to mouse IgG. After conjugation to the dye, the antibody was further purified by gel filtration, resulting in a highly pure and specific antibody. Hypermol® secondary antibodies are highly specific and purified to remove unbound dye, minimizing background signal. |
| Storage instructions | Store as glycerol stock at -20°C. Avoid repeated freeze/thaw cycles. |
| Shipping conditions | Shipped at ambient temperature. |
| Remarks | For Use in Research only. Not for Use in Human or Veterinary Diagnostical or Therapeutical Applications. |
Buffers and antibodies from Hypermol® are made of the ultrapure reagents in Milli-Q™ water, as described in our publications.
Further Information
Product DataSheet
Material and Safety Data Sheet
References
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Physiologic and Nanoscale Distinctions Define Glutamatergic Synapses in Tonic vs Phasic Neurons.
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J Neurosci. 2023;43(25):4598-4611. doi:10.1523/JNEUROSCI.0046-23.2023.
Bitbow Enables Highly Efficient Neuronal Lineage Tracing and Morphology Reconstruction in Single Drosophila Brains.
Li Y, et al.
Front Neural Circuits. 2021;15:732183. doi:10.3389/fncir.2021.732183.
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