Talin (smooth muscle, turkey) - 1.0 mg

Protein

Talin (Talin-1)

Origin

Smooth muscle, turkey

Molecular mass

~270kDa

Protein description

Talin isolated from smooth muscle has a molecular mass of ~270kD. Talin consists of a 220kD rod containing actin, vinculin and integrin binding sites and an N-terminal 47kD head region with a FERM domain.
The actin-binding multiligand protein Talin interacts e.g. with phospholipids, signaling molecules, and cytoskeletal components and is regarded as one of the key components of intracelular focal adhesion assembly.

Actin interaction

Actin binding protein.

Form

Lyophilized, ready-to-use.

Quantity per unit

20x50 µg (1.0 mg)

Buffer

0.1M KCl, 10mM imidazole pH 7.4, 2mM MgCl2, 0.2mM DTT and 2% disaccharides, when each tube is reconstituted with 50 µl ultrapure water to obtain a 1mg/ml solution.

Purity

~92% by scanning densitometry from Coomassie G-250 stained SDS-Gels.

Purification notes

Purified from turkey gizzard muscle.

Protein concentration

Determined by Biuret method.

Storage instructions

Upon reconstitution the product is stored on ice. This product can also be stored as a glycerol stock at -20°C. Avoid repeated freeze / thaw cycles.

Shipping conditions 

At ambient temperature. Upon delivery store at -70°C.

Remarks

For Use in Research only. Not for Use in Human or Veterinary Diagnostical or Therapeutical Applications. 

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