TMR-Actin (alpha-actin, rabbit skeletal muscle) - 5 x 100 µg

Protein

TMR-Actin

Origin

Skeletal muscle alpha actin, rabbit

Molecular mass

42kDa

Protein description

G-actin from rabbit skeletal muscle is a single chain polypeptide with a molecular weight of 42kD consisting of 375 amino acids. TMR-Actin is chemically modified G-actin, carrying a tetramethlyrhodamine group covalently bound to Cys374 at the C-term of the molecule, rendering the actin unpolymerizable.

Conjugation ratio

TMR-Actin is supplied with a DOL=0.9-1 (DOL -Degree of labelling). 

Form

Lyophilized, ready-to-use.

Quantity 

per unit

5x 100 µg

Buffer

2mM Tris-Cl pH 8.2, 0.4mM ATP, 0.5mM DTT, 0.1mM CaCl2, 1mM NaN3 and 0.3% disaccharides, when reconstituted with 100 µl ultrapure water to each tube to obtain a 1mg/ml solution.

Purity

>99% by scanning densitometry.

Purification notes

Purified by GPC from rabbit skeletal muscle.

Protein concentration

Determined by the Biuret method.

Storage instructions

TMR-Actin is stored at –70°C upon arrival and will be stable in performance for at least 6 months from the date of purchase. Solubilized pyrene actin is kept on ice and should be used within 1 week. Avoid refreezing.    

Shipping conditions 

At ambient temperature. Upon delivery store at -70°C.

Remarks

CAS no.