Tropomyosin (cardiac Tropomyosin) - 2x100µg

Protein

Tropomyosin

Origin

cardiac muscle, porcine

Molecular mass

~70kDa

Molecular structure

Heterodimeric protein (α,β), consisting of alpha helical coiled coils, that binds laterally to the actin filament in muscle and non-muscle cells.

Actin interaction

Laterally binds to F-actin at a molar ratio of 1:7 (tropomyosin:g-actin).

Form

Lyophilized, ready-to-use.

Quantity per unit

2x100 µg

Buffer

0.05M KCl, 20mM imidazole pH 7.4, 1mM EDTA, 1mM DTT and 2.5% disaccharides, when reconstituted with ultrapure water to obtain a 1mg/ml solution.

Purity

>98% by scanning densitometry.

Purification notes

Purified from porcine cardiac muscle.

Protein concentration

Determined by the Biuret method.

Storage instructions

Upon reconstitution the product is stored on ice. This product can also be stored as a glycerol stock at -20°C. Avoid repeated freeze / thaw cycles.

Shipping conditions 

At ambient temperature. Upon delivery store at -70°C.

Remarks

For Use in Research only. Not for Use in Human or Veterinary Diagnostical or Therapeutical Applications.

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