Tropomyosin (smooth muscle) - 1 mg
Actin Binding Protein
Tropomyosin
(Actin Binding Protein)
Cat. #: 8309-02
Description
| Protein | Tropomyosin |
| Origin | Smooth muscle, turkey |
| Molecular mass | ~70 kDa |
| Molecular structure | Heterodimeric protein (α, β), consisting of alpha helical coiled coils, that binds laterally to the actin filament in muscle and non-muscle cells. |
| Actin interaction | Laterally binds to F-actin at a molar ratio of 1:7 (tropomyosin:g-actin). |
Buffers and antibodies from Hypermol® are made of the ultrapure reagents in Milli-Q™ water, as described in our publications.
Properties
| Form | Lyophilized, ready-to-use. |
| Quantity per unit | 1.0 mg |
| Buffer | 0.05M KCl, 20mM imidazole pH 7.4, 1mM EDTA, 1mM DTT and 2.5% disaccharides, when reconstituted with ultrapure water to obtain a 1mg/ml solution. |
| Purity | >96% by scanning densitometry. |
| Purification notes | Purified from turkey gizzard |
| Protein concentration | Determined by the Biuret method. |
| Storage instructions | Upon reconstitution the product is stored on ice. This product can also be stored as a glycerol stock at -20°C. Avoid repeated freeze / thaw cycles. |
| Shipping conditions | At ambient temperature. Upon delivery store at -70°C. |
| Remarks | For Use in Research only. Not for Use in Human or Veterinary Diagnostical or Therapeutical Applications. |
| CAS no. | |
Buffers and antibodies from Hypermol® are made of the ultrapure reagents in Milli-Q™ water, as described in our publications.
Further Information
References
Cooperative effects of tropomyosin on the dynamics of the actin filament.
Khaitlina S, Tsaplina O, Hinssen H. FEBS Lett. 2017 Jul;591(13):1884-1891. doi: 10.1002/1873-3468.12700. Epub 2017 Jun 11.
Khaitlina S, Tsaplina O, Hinssen H. FEBS Lett. 2017 Jul;591(13):1884-1891. doi: 10.1002/1873-3468.12700. Epub 2017 Jun 11.
Construction of an atomic model for tropomyosin and implications for interactions with actin.
Phillips Jr GN. J.Mol.Biol. (1986) 192 p.128.
Phillips Jr GN. J.Mol.Biol. (1986) 192 p.128.
Product DataSheet
Material and Safety Data Sheet
Protein Sequence on NCBI (all sequences)Protein Structure on NCBI
Cooperative effects of tropomyosin on the dynamics of the actin filament.
Khaitlina S, Tsaplina O, Hinssen H.
FEBS Lett. 2017 Jul;591(13):1884-1891. doi: 10.1002/1873-3468.12700. Epub 2017 Jun 11.
J.Mol.Biol. (1986) 192 p.128
