Protein / Peptide

Vinculin binding site of talin

Origin

Synthetic

Molecular mass

2.9 kDa

Description

Vinculin is autoinhibited by the head/tail interaction of the protein. This inhibition can be released by several ligands, such as PIP2 and talin. VBS is a peptide which derives from the C-terminal talin sequence and was shown to release the head/tail interaction of vinculin by binding to the vinculin head (Vh). The vinculin molecule opens and exposes e.g. ligand binding sites in the vinculin tail (Vt) region.

PIP2 and actin binding have been shown to be mutually exclusive (Steimle et al., 1999), while binding of VBS opens the vinculin molecule without impairing actin binding (Bass et al., 2002). The activation of vinculin by talin to connect the actin cytoskeleton to the plasma membrane is thought to be one of the key mechanisms for focal adhesion assembly.

Actin interaction

Vinculin binding peptide to activate vinculin for actin binding. 

Form

Lyophilized, ready-to-use.

Quantity  per unit

200 µg

Buffer

Ultrapure water; add 200µl to obtain a 1mg/ml solution.

Purity

>98% by HPLC.

Molar ratio for activation

A molar ratio of 30:1 (VBS:Vinculin) is required to open vinculin or metavinculin (Witt et al., 2004). Hence, 100µg of VBS are sufficient to activate 133µg of vinculin at this given molar ratio or less if higher excess is used .

Protein concentration

Determined amino acid analysis.

Storage instructions

VBS is stored at –70°C upon arrival and will be stable in performance for at least 1 year from date of purchase. The solubilized peptide should be aliquoted and refrozen. If kept on ice and it should be used within 2 weeks. 

1-2 freeze / thaw cycles of aliquots is possible.

Shipping conditions 

At ambient temperature. Upon delivery store at -70°C.

Remarks

For Use in Research only. Not for Use in Human or Veterinary Diagnostical or Therapeutical Applications. 

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