VBS
(Vinculin activating peptide)
Cat. #: 8317-02
.............................................................................................................................................................................................................................................................................................................................................................................................................
Description
|
|
Protein / Peptide
|
Vinculin binding site of talin
|
Origin
|
Synthetic
|
Molecular mass
|
2.9 kDa
|
Description
|
Vinculin is autoinhibited by the head/tail interaction of the protein. This inhibition can be released by several ligands, such as PIP2 and talin. VBS is a peptide which derives from the C-terminal talin sequence and was shown to release the head/tail interaction of vinculin by binding to the vinculin head (Vh). The vinculin molecule opens and exposes e.g. ligand binding sites in the vinculin tail (Vt) region.
PIP2 and actin binding have been shown to be mutually exclusive (Steimle et al., 1999), while binding of VBS opens the vinculin molecule without impairing actin binding (Bass et al., 2002). The activation of vinculin by talin to connect the actin cytoskeleton to the plasma membrane is thought to be one of the key mechanisms for focal adhesion assembly.
|
Actin interaction
|
Vinculin binding peptide to activate vinculin for actin binding.
|
Properties
|
|
Form
|
Lyophilized, ready-to-use.
|
Quantity per unit
|
1.0 mg
|
Buffer
|
Ultrapure water; add 1.0 ml to obtain a 1mg/ml solution.
|
Purity
|
>98% by HPLC.
|
Molar ratio for activation
|
A molar ratio of 30:1 (VBS:Vinculin) is required to open vinculin or metavinculin (Witt et al., 2004). Hence, 100µg of VBS are sufficient to activate 133µg of vinculin at this given molar ratio or less if higher excess is used .
|
Protein concentration
|
Determined amino acid analysis.
|
Storage instructions
|
VBS is stored at –70°C upon arrival and will be stable in performance for at least 1 year from date of purchase. The solubilized peptide should be aliquoted and refrozen. If kept on ice and it should be used within 2 weeks.
1-2 freeze / thaw cycles of aliquots is possible.
|
Shipping conditions
|
At ambient temperature. Upon delivery store at -70°C.
|
Remarks
|
For Use in Research only. Not for Use in Human or Veterinary Diagnostical or Therapeutical Applications.
|
CAS no.
|
|
Further Information
Product DataSheet
Material and Safety Data Sheet
Protein Sequence on NCBI
Phosphoinositides regulate force-independent interactions between talin, vinculin, and actin.
Kelley CF, Litschel T, Schumacher S, Dedden D, Schwille P, Mizuno N. , Elife. 2020;9:e56110. Published 2020 Jul 13. doi:10.7554/eLife.56110
Polyphosphoinositides inhibit the interaction of vinculin with actin filaments.
Steimle PA, Hoffert JD, Adey NB, Craig SW., J Biol Chem. 1999 Jun 25;274(26):18414-20.
Further characterization of the interaction between the cytoskeletal proteins talin and vinculin.
Bass MD, Patel B, Barsukov IG, Fillingham IJ, Mason R, Smith BJ, Bagshaw CR, Critchley DR., Biochem J. 2002 Mar 15;362:761-8.
Comparative biochemical analysis suggests that vinculin and metavinculin cooperate in muscular adhesion sites.
Witt S, Zieseniss A, Fock U, Jockusch BM, Illenberger S., J Biol Chem. 2004 Jul 23;279(30):31533-43. Epub 2004 May 24.
Structural basis for vinculin activation at sites of cell adhesion.
Bakolitsa C, Cohen DM, Bankston LA, Bobkov AA, Cadwell GW, Jennings L, Critchley DR, Craig SW, Liddington RC. Nature. 2004 Jul 29;430(6999):583-6. Epub 2004 Jun 13.