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Anti-Myc ATTO565 (clone 9E10)

Cat. #:
7904-01
Shipping:
FedEx Express (Global) FedEx Express (Global) (shipping info)
Quantity:
200 µg



Anti-Myc ATTO565
clone 9E10, >96% purity 


Cat. # 7904-01

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Description

   

Product name

Optical properties

Anti-Myc  ATTO565 (clone 9E10)

λex 563nm, λem 592nm 


Target 

Immunogen :  synthetic peptide corresponding to aa 408-439 from C-terminus of human c-myc.
Sequence : Epitope sequence, EQKLISEEDL (aa 410-419 of human c-myc protein).

Description

The Myc Tag is an epitope tag, commonly used to localize gene products in many types of cultured cells, to study the topology of proteins and protein complexes. Anti-Myc is especially useful whenever no primary antibodies against the proteins of interest are available, mostly because they are less immunogenic and low abundant.
 
Monoclonal Anti-Myc is directed against a peptide epitope of the human proto-oncogene p62c-myc. The immunogen corresponds to amino acids 408-439 of the Leucine zipper region of human c-Myc (AEEQKLISEEDLLRKRREQLKHKLEQLRNSCA). Anti-Myc recognizes the epitope EQKLISEEDL.
 
The antibody is used for the immunodetection and functional characterization of Myc and recombinant proteins containing the Myc epitope tag. It is frequently used for the detection and characterization of recombinant expressed Myc-tagged proteins in vivo and in vitro or proteins containing the epitope EQKLISEEDL. Anti-Myc facilitates protein detection in all blotting techniques and immunofluorescence & -chemistry of tagged proteins.The Myc Tag is an epitope tag, commonly used to localize gene products in many types of cultured cells, to study the topology of proteins and protein complexes. Anti-Myc is especially useful whenever no primary antibodies against the proteins of interest are available, mostly because they are less immunogenic and low abundant.
 
Monoclonal Anti-Myc is directed against a peptide epitope of the human proto-oncogene p62c-myc. The immunogen corresponds to amino acids 408-439 of the Leucine zipper region of human c-Myc (AEEQKLISEEDLLRKRREQLKHKLEQLRNSCA). Anti-Myc recognizes the epitope EQKLISEEDL.

 
The antibody is used for the immunodetection and functional characterization of Myc and recombinant proteins containing the Myc epitope tag. It is frequently used for the detection and characterization of recombinant expressed Myc-tagged proteins in vivo and in vitro or proteins containing the epitope EQKLISEEDL. Anti-Myc facilitates protein detection in all blotting techniques and immunofluorescence & -chemistry of tagged proteins.

Specification

Immunofluorescence (IF) . 2-10 µg/mL
Immunohistochemistry (Paraffin) (IHC (P)) : 1:100
Immunoprecipitation (IP) : 2-5 µg
ELISA (ELISA) .: 0.1-1.0 µg/mL
Western Blot (WB) : 1-2 µg/mL

Synonyms


Proto-oncogene c-Myc; Transcription factor p64; Class E basic helix-loop-helix protein 39; BHLHE39; c-myc 



Properties

   

Form

Freeze-dried..

Quantity

200 µg

Product content
 

200 µg Anti-Myc ATTO565
0.5 ml Glycerol-PBS (Reconstitution Buffer)
Product DataSheet

Clonality

Monoclonal

Isotype

Mouse IgG1, kappa

Storage instructions

Store as glyecrol stock at -20°C, Avoid freeze/thaw cycles.

Remarks

 

CAS no.

For Use in Research only. Not for Use in Human or Veterinary Diagnostical or Therapeutical Processes. 

 



Further Information 


Product DataSheet

Material and Safety Data Sheet

Protein Sequence on NCBI

 



References

Evan GI, Lewis GK, Ramsay G, Bishop JM. Isolation of monoclonal antibodies specific for human c-myc proto-oncogene product. Mol Cell Biol. 1985 Dec;5(12):3610-6. PMID:3915782