G-Actin Buffers

 
 
Hypermol buffer reagents
G-Actin buffer systems

Fresh buffer conditions for reliable actin performance

Ready-to-reconstitute G-actin buffers for monomeric actin handling, ligand dilution and dialysis workflows.

Suitable buffer composition is essential for preserving protein state and assay reproducibility. Hypermol® G-actin buffers are prepared from ultrapure reagents in Milli-Q™ water and supplied as convenient dry formulations for fresh preparation at the bench.

Use
Dilution, dialysis and ligand handling for G-actin workflows.
Format
Lyophilized stocks for fresh reconstitution with ultrapure water.
Workflow
Designed for convenient, time-saving bench preparation.
MonoMix is used to dilute G-actin, dialyse G-actin or ligands, and maintain buffer conditions for monomeric actin. G-actin itself is supplied in MonoMix, providing compatible starting conditions for downstream actin experiments.
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Buffers from Hypermol® are made of purest reagents in Milli-Q™ water, as described in our publications.
G-actin buffer
NEW
MonoMix - 100 ml (G-Actin buffer with 1mM DTT)
MonoMix is a G-actin buffer for dilution of G-actin or G-actin ligands, used:
  • to maintains the G-actin state;
  • for dialysis of actin and ligands in actin assays;
Units:
1x
42,00 EUR
excl. Shipping costs
G-actin buffer
MonoMix 5x 100 ml (G-Actin buffer with 1mM DTT)
MonoMix is a G-actin buffer for dilution of G-actin or G-actin ligands, used:
  • to maintains the G-actin state;
  • for dialysis of actin and ligands in actin assays;
Units:
1x
124,00 EUR
excl. Shipping costs
MonoMix .01
MonoMix .01 - 100ml (G-actin buffer with .01mM DTT)
MonoMix .01 is a G-actin buffer for dilution of G-actin or G-actin ligands, used:
  • to maintains the G-actin state;
  • for dialysis of actin and ligands in actin assays;
  • for use in DTT-sensitiv assays;
Quantity:
2x1.0 ml (50x)
Units:
1x
5x ( +72,00 EUR )
48,00 EUR
excl. Shipping costs
ATP Actin Buffer , 1L (10x G-Actin buffer )
ATP Actin Buffer , 1L (10x G-Actin buffer )
ATP Actin Buffer is a G-actin buffer to dialyse G-actin and/or G-actin ligands. 

Essential G-actin buffer to:
 
  • maintains the G-actin state;
  • used for dialysis of actin and ligands in actin assays;
Quantity:
2x1.0 ml (50x)
Units:
1x
5x ( +294,00 EUR )
195,00 EUR
excl. Shipping costs
ME Buffer - 10x Mg-Exchange Buffer (4x0.5ml)
ME Buffer - 10x Mg-Exchange Buffer (4x0.5ml)
Buffer to convert Ca-ATP actin to Mg-ATP actin.
44,00 EUR
excl. Shipping costs
MonoMix .1 (50x G-actin buffer with .1mM DTT)
MonoMix .1 (50x G-actin buffer with .1mM DTT)
MonoMix is a G-actin buffer for dilution of G-actin or G-actin ligands used:
  • to maintain the G-actin state;
  • for dialysis of actin and ligands in actin assays with 0.1mM DTT;
Quantity:
2x1.0 ml (50x)
Units:
1x
5x ( +72,00 EUR )
78,00 EUR
excl. Shipping costs
CS-LSB (Cytoskeleton Low Salt Buffer)
CS-LSB (Cytoskeleton Low Salt Buffer)
CS-LSB is used to:
  • Washing and low salt buffering of cell lysate derived preparations;
Quantity:
30ml
Units:
1x
5x ( +284,00 EUR )
119,00 EUR
excl. Shipping costs
GAB-P
GAB-P
GAB-P is used for Biacore SPR to:
  • support ligand binding to G-actin;
  • maintain the G-state of actin;
  • prevent denaturing of actin;
Quantity:
250ml
Units:
1x
67,00 EUR
excl. Shipping costs
MonoMix .01
NEW
MonoMix .01 - 5x 100ml (G-actin buffer with .01mM DTT)
MonoMix .01 is a G-actin buffer for dilution of G-actin or G-actin ligands, used:
  • to maintains the G-actin state;
  • for dialysis of actin and ligands in actin assays;
  • for use in DTT-sensitiv assays;
Quantity:
2x1.0 ml (50x)
Units:
1x
5x ( +72,00 EUR )
132,00 EUR
excl. Shipping costs
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