MonoMix .01
G-Actin buffer with ATP and 0.1mM DTT
Cat. # 5120-01
...............................................................................................................................................................................................................................................................................................................................................................................................................
Applications
G-actin buffer for applications sensitive to reducing agents. Used to dilute G-actin or G-actin ligands;
Description
|
|
Product name
|
MonoMix .01
|
Product description
|
MonoMix .01 is a G-actin buffer with less DTT compared to MonoMix (Cat.# 5100-01), containing 1mM DTT. The buffer is used to dilute G-actin or G-actin ligands. Actin from Hypermol is supplied in MonoMix and can be reconstituted simply by the addition of H2O. To further dilute G-actin, either MonoMix .01 or a freshly made buffer containing the components listed below should be used.
|
Overview
|
Essential G-actin buffer to:
- maintains the G-actin state;
- used for dialysis of actin and ligands in actin assays;
- for use in DTT-sensitive assays;
|
Properties
|
|
Form
|
Lyophilized, ready-to-use.
|
Quantity
|
2x1ml 50x stock (2x aliquots to reconstitute with 1.0 ml ultrapure water to obtain a 50x stock per aliquot)
|
Buffer
|
2mM Tris-Cl pH 8.2, 0.4mM ATP, 0.1mM CaCl2 and 0.01mM DTT, when reconstituted with 50 ml ultrapure water.
|
Purity
|
Ultrapure reagents.
|
Storage instructions
|
MonoMix .01 is stored at –70°C upon arrival and will be stable in performance for at least 6 months from the date of purchase. Solubilized MonoMix .01 is kept on ice and should be used within 1 week. Avoid refreezing.
|
Shipping conditions
|
At ambient temperature. Upon delivery store at -70°C.
|
Remarks
|
For Use in Research only. Not for Use in Human or Veterinary Diagnostical or Therapeutical Applications.
|
CAS no.
|
|
Further Information
Product DataSheet
Material and Safety Data Sheet
Buffers from Hypermol are made of purest reagents in Milli-Q™
water, as described in our publications.
Validated product quality with Satisfaction Guarantee!