Actin-Toolkits
Detection and Analyses of Actin Interactions
Ready-to-use for instant, reliable results!
Actin-Toolkits are designed for a spectrum of applications: from fast verification of biological activity of a putative actin-binding protein to comprehensive assay sets for biochemical and structural characterization of ligand–actin interactions.
Fail-safe handling of actin assays
Defined components and workflows reduce variability and support robust assay execution.
Saving your time
All critical reagents in one kit—focus on data generation rather than preparation.
Maximizing your research efforts
From interaction screening to kinetics, imaging, and structural readouts—choose the depth you need.
Actin-Toolkits utilize actin as a tool for functional and structural analysis of ligands with actin-binding sites. They are based on established in vitro and in situ methods, and remain convenient for experienced researchers designing their own experimental setups.
Actin-Toolkit F-Actin Binding
Investigate ligand interactions with filaments, including bundling and network formation.
Actin-Toolkit TIRFM
Complete ready-to-use kit for high- and super-resolution imaging (e.g., single-molecule TIRF, in-vitro motility assays).
Actin-Toolkit Fluorescence Microscopy
Next-generation fluorescent actin: strong fluorescent Atto-Actins, ready-to-start for visualization of interactions.
Actin-Toolkit G-Actin Binding
Proof binding of ligands to G-actin using G-actin conjugated to ActinBeads for simple low-speed precipitation workflows.
Actin-Toolkit Fluorometry
Determine polymerization and depolymerization by measuring fluorescence of pyrenyl-conjugated actin; analyze ligands affecting actin dynamics.
Actin-Toolkit SPR
Unique method for kinetic and affinity analysis of ligand interactions with actin filaments (F-actin ligands).
Actin-Toolkit ELISA
Direct or indirect ELISA assays for ligand interactions with G-actin or F-actin; compatible with purified ligands through crude extracts.
Actin-Toolkit Crystallography
Smart crystallization of G-actin alone or in complex with ABPs using Non-Polymerizing Actin as a practical alternative to chemically modified actins.
Actin-Toolkit Microinjection
Inject fluorescent actin into cultured cells to visualize intracellular actin dynamics and enable co-localization studies with actin-binding proteins.
Actin-Toolkit Electron Microscopy
Ready-to-use preparation of negatively stained specimens for EM analysis of proteins, complexes, and networks on G- and F-actin.
| Toolkit | Primary readout | Typical question | Link |
|---|---|---|---|
| F-Actin Binding | Binding to filaments; bundling/network formation | Does my ligand bind F-actin and remodel filaments? | Open |
| TIRFM | High/super-resolution imaging workflows | Single-molecule behavior, motility, high-resolution interaction readouts? | Open |
| Fluorescence Microscopy | Atto-actin based visualization | Where/when does interaction occur in fluorescence imaging? | Open |
| G-Actin Binding | ActinBeads precipitation assay | Does my ligand bind monomeric (G) actin? | Open |
| Fluorometry | Pyrenyl-actin fluorescence kinetics | How do ligands affect polymerization/depolymerization? | Open |
| SPR | Kinetics & affinity for F-actin ligands | What are on/off rates and affinity to F-actin? | Open |
| ELISA | Direct/indirect ELISA (G- or F-actin) | Quantify actin binding across concentrations and sample types? | Open |
| Crystallography | Complex crystallization with Non-Polymerizing Actin | Structural analysis of actin–ligand complexes? | Open |
| Microinjection | Cellular incorporation of fluorescent actin | Visualize intracellular actin dynamics and co-localization? | Open |
| Electron Microscopy | Negative staining EM specimen prep | High magnification imaging of filaments/complexes/networks? | Open |
Need small quantities for a fast feasibility check?
Actin-Trialkits are complete ready-to-use mini-applications for testing and screening when minor quantities are required.
Proteins from Hypermol are of validated high biological activity, as described in our publications.
Validated product quality with Satisfaction Guarantee!
Hypermol – Kits to Analyse Actin Interactions
Hypermol offers high quality products for research. Find a variety of kits to work with actin and actin binding proteins in the Actin Toolkits section. Actin Toolkits are all-in-one solutions, for various methods and applications, to analyse your actin binding ligand. Each kit comes with a manual, that guides you through the work with actin. And should you have a question, simply contact our dedicated customer service for support.
Actin kits where developed to save your time and maximize the output of your efforts.
Actin Assays and Kits – Multiple Uses and Applications in Research
Actin Toolkits from Hypermol are used for safe and reliable testing of G- or F- actin interactions with actin-binding proteins. Actin-Toolkits utilize actin as a tool for the functional and structural analysis of ligands with actin binding sites. Actin-Toolkits are based on established in vitro and in situ methods. They are also convenient to use for experienced researchers who wish to design their own methods.
Actin-Toolkits are all-in-one applications for:
- fail-safe handling of actin assays,
- saving your time and
- maximizing your research efforts.
Actin Toolkits from Hypermol – Learn More About It!
If you have more questions about use and applications of Actin Toolkits. Or would you like to know, what you can achieve with them or learn more technical details?
Then you should contact us and get acquainted with our customer service, who happily welcomes your query 24/7 under cusserv@hypermol.com.
More Than 99% of All Products in Storage
For all who already found their favourite products, the Hypermol e-shop is open 24/7 to place your order.
We usually have more than 99% of all products in storage and ship same day if the order is placed until 14:00 EST to any destination worldwide.
The distributors of Hypermol products in Asia will be happy to receive your request.
Hypermol is your expert for actin assays and kits, with a great variety of products. For you, to be ahead in research!