Actin-Toolkit Microinjection (ATTO565-Actin, skeletal muscle actin)

Protein

ATTO565-Actin

Origin

Skeletal muscle alpha actin, rabbit

Molecular mass

42kDa

Toolkit description

The Actin-Toolkit Microinjection all-in-one kit contains rhodamine conjugated α-actin to be microinjected into either normal or transfected cells. For this purpose purified actin was covalently lysine labeled at ATTO565 , leaving the polymerization properties of actin essentially unimpaired. 

The Actin-Toolkit Microinjection contains 4x100µg ATTO565-Actin, rhodamine conjugated BSA as a reference, ATP-buffers suited for microinjection of actin and mini filter units for the convenient preparation of injectable actin. A handbook is provided with the kit, giving a detailed description of the use of actin for the microinjection technique for a safe and error-free handling of ATTo565 actin.

Purity

>99% by scanning densitometry.

Form

Lyophilized, ready-to-use.

Kit content

4x100µg ATTO565-Actin (lyophilized powder);
1x50µg Rhodamine-BSA (lyophilized powder);
5x50ml MonoMix .01 (lyophilized G-actin ATP-buffer with 0.01mM DTT);
5x100µl Syringe Filter Units 0.22µM;
1x Toolkit Handbook Microinjection;

Actin Buffer

2mM Tris-Cl pH 8.2, 0.4mM ATP, 0.5mM DTT, 0.1mM CaCl2, 1mM NaN3 and 0.3% disaccharides, when reconstituted with 100µl ultrapure water to obtain a 1mg/ml solution.

DOL

>0.5

Purification notes

Purified from rabbit skeletal muscle, GPC.

DOL

Determined by the Biuret method.

Storage instructions

We guarantee stable performance of the kit components for 6 months when stored at -70°C upon arrival. Solubilized G-actin is kept on ice and should be used within 1 week. Avoid refreezing.     

Shipping conditions 

At ambient temperature. Upon delivery store at -70°C.

Remarks

For Use in Research only. Not for Use in Human or Veterinary Diagnostical or Therapeutical Applications. 

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