Actin-Toolkit Microinjection (Rhodamine-Actin, skeletal muscle actin)

Protein

Rhodamine-Actin

Origin

Skeletal muscle alpha actin, rabbit

Molecular mass

42kDa

Toolkit description

The Actin-Toolkit Microinjection all-in-one kit contains rhodamine conjugated α-actin to be microinjected into either normal or transfected cells. For this purpose purified actin was covalently labeled at Cys 374 with tetramethylrhodamine, leaving the polymerization properties of actin essentially unimpaired. 

The Actin-Toolkit Microinjection contains 5x100µg Rhodamine-Actin (Rh-Actin), rhodamine conjugated BSA as a reference, ATP-buffers suited for microinjection of actin and mini filter units for the convenient preparation of injectable actin. A handbook is provided with the kit, giving a detailed description of the use of actin for the microinjection technique for a safe and error-free handling of Rhodamine-Actin.

Purity

>99% by scanning densitometry.

Form

Lyophilized, ready-to-use.

Kit content

5x100µg Rhodamine-Actin (lyophilized powder);
1x50µg Rhodamine-BSA (lyophilized powder);
5x50ml MonoMix .01 (lyophilized G-actin ATP-buffer with 0.01mM DTT);
5x100µl Syringe Filter Units 0.22µM;
1x Toolkit Handbook Microinjection;
5x Black Tubes & Tags;

Actin Buffer

2mM Tris-Cl pH 8.2, 0.4mM ATP, 0.5mM DTT, 0.1mM CaCl2, 1mM NaN3 and 0.3% disaccharides, when reconstituted with 100µl ultrapure water to obtain a 1mg/ml solution.

DOL

>0.5

Purification notes

Purified from rabbit skeletal muscle, GPC.

DOL

Determined by the Biuret method.

Storage instructions

We guarantee stable performance of the kit components for 6 months when stored at -70°C upon arrival. Solubilized G-actin is kept on ice and should be used within 1 week. Avoid refreezing.     

Shipping conditions 

At ambient temperature. Upon delivery store at -70°C.

Remarks

For Use in Research only. Not for Use in Human or Veterinary Diagnostical or Therapeutical Applications. 

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