F-Actin Buffers

 
 
Hypermol buffer reagents
F-Actin buffer systems

Fresh buffer conditions for actin filament work

Ready-to-reconstitute F-actin buffers with short shelf-life components for reliable polymerization and filament handling.

F-actin buffer performance depends on freshly prepared conditions, especially when ATP and DTT are required. Hypermol® F-actin buffers are prepared from ultrapure reagents in Milli-Q™ water and supplied as convenient dry formulations for time-saving bench work.

Use
Polymerization, dilution and ligand handling for F-actin workflows.
Format
Dry buffer formulations for fresh reconstitution with ultrapure water.
Components
Includes sensitive components such as ATP and DTT for fresh preparation.
PolyMix is supplied as a one-step 10x stock after reconstitution: add 1 ml of ultrapure water and mix 1:10 v/v with G-actin. PolyMix is used to dilute F-actin and ligands, providing suitable conditions for in vitro work with actin filaments.
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Buffers from Hypermol® are made of purest reagents in Milli-Q™ water, as described in our publications.
KMEI Buffer (10x) - 4x1.0ml
Multipurpose buffer to:
  • use for many cytoskeletal proteins;
  • to polymerize actin;
Quantity:
2x1.0ml
Units:
1x
2x ( +16,00 EUR )
51,00 EUR
F-Actin BufferKit (to modulate actin dynamics)
F-Actin BufferKit is used to:
  • polymerize G-actin to F-actin;
  • manipulate actin dynamics by varying the salt conditions;
  • prevent denaturing of actin during analysis;
Units:
1x kit
79,00 EUR
CS-HSB (Cytoskeleton High Salt Buffer)
CS-HSB is used to:
  • treatment of cell lysate derived preparations under highly stringent conditions;
Quantity:
30ml
Units:
1x
5x ( +332,00 EUR )
121,00 EUR
CS-LB (Cytoskeleton LysisBuffer)
CS-LB is used to:
  • prepare cell lysates for cytoskeleton research;
  • support the stability of cytoskeletal proteins;
  • prevent proteolytic degradation; 
Quantity:
10 x 1.5 ml
Units:
1x
5x ( +360,00 EUR )
156,00 EUR
CS-LSB (Cytoskeleton Low Salt Buffer)
CS-LSB is used to:
  • Washing and low salt buffering of cell lysate derived preparations;
Quantity:
30ml
Units:
1x
5x ( +284,00 EUR )
119,00 EUR
CS-MSB (Cytoskeleton Medium Salt Buffer)
CS-MSB is used to:
  • washing and buffer cell lysate derived preparations;
  • support the stability of cytoskeletal proteins; 
Quantity:
50ml
Units:
1x
5x ( +286,00 EUR )
119,00 EUR
FAB-P
FAB-P is used for Biacore SPR to:
  • support ligand binding to F-actin;
  • maintain the filamentous state of actin;
  • prevent denaturing of actin;
Quantity:
250ml
Units:
1x
72,00 EUR
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