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Actin Conjugates

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State of the art techniques rely on actin conjugates to quantify actin dynamics, map actin–ligand interactions and enable fluorescence-based readouts across microscopy, fluorometry and biochemical assays. Hypermol offers a focused portfolio of actin conjugates with defined coupling chemistries and application-optimized formats.

We exclusively manufacture the novel ATTO-Actins , designed for demanding fluorescence microscopy workflows, including high-resolution and single-molecule imaging (TIRF, STED, etc.).

Fluorescence microscopy

ATTO / Rhodamine readouts for filament architecture and dynamics.

Single-molecule / TIRFM

TIRFM series for high-contrast surface-near imaging.

Fluorometry / kinetics

Pyrene-actin for polymerization kinetics and binding studies.

Biochemical interfaces

Biotin / NBD / TMR formats for defined interaction assays.

Conjugate Selector

ATTO-Actin (skeletal muscle) Chemistry: lysine esterification (activated ATTO dyes). Use: broad fluorescence microscopy and imaging workflows.	ATTO-Actin (cardiac muscle) Chemistry: lysine esterification (activated ATTO dyes). Use: fluorescence imaging with cardiac actin context.	ATTO-Actin (non-muscle) Chemistry: lysine esterification (activated ATTO dyes). Use: fluorescence imaging with non-muscle actin context.
ATTO-Actin for TIRFM (skeletal) Application series: TIRF microscopy and related high-resolution methods. Format: ready-to-use fluorescent actin conjugates.	ATTO-Actin for TIRFM (non-muscle) Application series: TIRF microscopy and single-molecule imaging workflows. Format: ready-to-use fluorescent actin conjugates.	ATTO Non-Polymerizing Actin Application: biophysical/biochemical characterization of G-actin binding proteins. Format: instantly soluble, ready-to-use modified actin.
Biotin-Actin Chemistry: lysine labeling with activated biotin esters (non-cleavable spacer arm). Use: in-vitro interaction assays (ELISA, motility, pull-down, microinjection).	Pyrene-Actin Chemistry: covalent pyrenyl group at Cys374 (controlled dye/protein ratios). Use: fluorometry-based characterization of actin dynamics.	Rhodamine-Actin Chemistry: lysine labeling via activated dye esters. Use: microscopy and actin structure visualization workflows.
NBD-Actin Chemistry: NEM conjugation followed by NBD coupling to Lys372. Use: actin–monomer interaction studies.	TMR-Actin Chemistry: tetramethylrhodamine-5-maleimide at Cys374. Use: monomeric, unpolymerizable actin for binding studies at high concentration.	Per product page Excitation/emission and degree of labeling (DOL) are listed per item to support rational dye selection and quantitative workflows.

Scientific Design Notes

ATTO series	Fluorescent actin conjugates prepared by esterification of lysine residues with activated ATTO dyes; validated to possess polymerization properties of native actin.
Biotin-actin	Lysine labeling with activated biotin esters; non-cleavable spacer arm supports detection and assay compatibility (ELISA, motility, pull-down, microinjection).
Pyrene-actin	Covalent pyrenyl group coupled at Cys374 with defined dye/protein ratios for fluorometry-based characterization of actin dynamics.
Rhodamine-actin	Lysine labeling via activated rhodamine esters; fluorescence microscopy readout for actin structures and dynamics.
NBD-actin	NEM conjugation followed by NBD coupling to Lys372; designed for analysis of actin–monomer interactions.
TMR-actin	Tetramethylrhodamine-5-maleimide labeling at Cys374; monomeric and unpolymerizable format for actin binding studies at elevated concentrations.

Proteins from Hypermol are of validated high biological activity, as described in our publications.

Validated product quality with Satisfaction Guarantee!

ATTO390-Actin (alpha-Actin, skeletal muscle rabbit) - 5x100µg

λ_ex 390nm, λ_em 479nm

Bright blue fluorescent skeletal muscle α-actin (rabbit), DOL= 0.5-0.8, >99% pure.

507,00 EUR