TMR-Actin (alpha-actin, rabbit skeletal muscle) - 2x 100 µg

Protein

TMR-Actin

Origin

Skeletal muscle alpha actin, rabbit

Molecular mass

42kDa

Protein description

G-actin from rabbit skeletal muscle is a single chain polypeptide with a molecular weight of 42kD consisting of 375 amino acids. TMR-Actin is chemically modified G-actin, carrying a tetramethlyrhodamine group covalently bound to Cys374 at the C-term of the molecule, rendering the actin unpolymerizable.
TMR actin is highly labelled to ensure the monomeric state of the TMR actin. We have validated TMR actin to be unpolymerizable up to concentrations  of 25mg/ml.  

Conjugation ratio

TMR-Actin is supplied with a DOL=0.9-1 (DOL -Degree of labelling). 

Form

Lyophilized, ready-to-use.

Quantity per unit

2x 100 µg

Buffer

2mM Tris-Cl pH 8.2, 0.4mM ATP, 0.5mM DTT, 0.1mM CaCl2, 1mM NaN3 and 0.3% disaccharides, when reconstituted with 100 µl ultrapure water to obtain a 1mg/ml solution.

Purity

>99% by scanning densitometry.

Purification notes

Purified by GPC from rabbit skeletal muscle.

Protein concentration

Determined by the Biuret method.

Storage instructions

TMR-Actin is stored at –70°C upon arrival and will be stable in performance for at least 6 months from the date of purchase. Solubilized pyrene actin is kept on ice and should be used within 1 week. Avoid refreezing.    

Shipping conditions 

At ambient temperature. Upon delivery store at -70°C.

Remarks

CAS no.