Actin-Toolkit Crystallography

 
Actin-Toolkit Crystallography
Non-Polymerizing Actin
Actin–ligand complexes
Ready-to-use crystallization system
 
Hypermol Actin-Toolkit Crystallography
Actin maintained in the monomeric state
Defined Non-Polymerizing Actin
Enzymatically modified α-skeletal muscle actin for structural studies requiring stable monomeric actin.
Structural analysis of actin complexes

Crystallization of actin and actin–ligand complexes

Non-Polymerizing Actin provides a defined alternative for structural studies in which spontaneous actin filament formation must be suppressed.

The Hypermol® Actin-Toolkit Crystallography is designed for crystallization of G-actin and co-crystallization of actin-binding proteins or other ligands with monomeric actin. Conventional G-actin can polymerize during experimental handling, complicating preparation of homogeneous actin–ligand complexes for crystallographic studies.

The toolkit therefore uses Non-Polymerizing Actin derived from rabbit skeletal muscle α-actin and enzymatically cleaved between Gly42 and Val43. This cleavage affects an essential monomer–monomer interaction region and suppresses polymerization in the presence of Ca2+, while the capacity of NP-Actin to interact with protein ligands remains essentially preserved. The system is suited to co-crystallization, stabilization of actin-binding ligands by complex formation and analysis of actin-dependent conformational changes.

Defined cleavage
α-skeletal muscle actin enzymatically cleaved between Gly42 and Val43.
Controlled assembly state
Polymerization is suppressed under Ca2+-actin conditions, facilitating stable monomeric preparations.
Ligand complexes
For structural studies of actin-binding proteins, domains, fragments and other actin ligands.
Browse Crystallography Toolkits
Controlled polymerization behavior
Non-Polymerizing Actin remains non-polymerized under Ca2+-actin conditions even in the presence of otherwise polymerizing salt concentrations. In the presence of Mg2+, the protein retains the capacity to form F-actin.
Actin source
Non-Polymerizing Actin is prepared from rabbit skeletal muscle α-actin and supplied at >99% purity.
Typical applications
Co-crystallization of actin-binding proteins with G-actin, stabilization of ligands through actin complex formation, structural analysis of actin–ligand complexes, investigation of conformational changes upon actin binding and biochemical interaction studies requiring monomeric actin.
Ready-to-use format
The toolkit combines Non-Polymerizing Actin with ATP-containing monomeric actin buffer and a detailed crystallography handbook for standardized experimental handling.
Hypermol Actin-Toolkit Crystallography
Maintain actin in a crystallization-compatible monomeric state.
NP-Actin provides a defined biochemical strategy for preparing actin–ligand complexes without relying on fluorescent or other bulky chemical modifications of the actin molecule.
Hypermol Satisfaction Guarantee
Validated product quality with Satisfaction Guarantee.
Actin products from Hypermol® are prepared and quality controlled for reliable biochemical and structural analysis of actin interactions.
 
Actin kit Crystallography
Actin-Toolkit Crystallography
  • Co-crystallization of actin binding proteins with Non-Polymerizable Actin;
  • Stabilization of ligands during crystallization by complex formation;
  • Analysis of conformational changes upon actin binding;
  • Suitable for biochemical applications;
3.895,00 EUR
excl. Shipping costs
1 to 1 (from a total of 1)