Arp2/3 & VCA-GST

Arp2/3 complex
Seven-subunit assembly
F-actin branching
VCA-activated nucleation
 
Hypermol Arp2/3 protein complex
The complex machinery
Native Arp2/3 complex for controlled actin nucleation, pointed-end capping and branched filament network formation.
Native actin nucleation machinery

Arp2/3 Protein Complex for actin nucleation and branched network formation

A native seven-subunit protein complex for VCA-activated actin nucleation, F-actin branching and reconstituted cytoskeleton assays.

The Arp2/3 Protein Complex is a central molecular machine for building branched actin networks. Upon activation by VCA-domain nucleation-promoting factors, the complex initiates new actin filaments from the side of pre-existing F-actin, generating characteristic branched filament architectures required for cell motility, membrane remodeling, endocytosis and intracellular transport.

Hypermol® Arp2/3 Protein Complex is natively prepared as a seven-subunit assembly with an apparent molecular mass of approximately 224 kDa. It is supplied as a highly purified, soluble and ready-to-use protein preparation for robust biochemical and cell-biological actin research.

Branch nucleation
Generates new daughter filaments from existing actin filaments after VCA activation.
Network architecture
Enables controlled formation of dense branched actin arrays in vitro.
Native complex
Seven-subunit assembly for authentic actin nucleation and branching workflows.
Browse Arp2/3 Protein Complex
For reconstituted branching assays
Use Arp2/3 together with actin, ATP and VCA-domain activators to build branched actin networks under defined biochemical conditions.
For cell motility research
Arp2/3-driven branching is a key mechanism behind lamellipodial actin networks, membrane protrusion and force generation at the cell edge.
For endocytosis and trafficking models
The complex is useful for studying actin-dependent membrane remodeling, vesicle movement and intracellular transport processes.
Strong experimental use cases
Reconstitute branched actin networks from purified components.
Measure VCA-dependent nucleation and branching activity.
Compare Arp2/3 activity with nucleators, capping proteins and actin binders.
Model lamellipodial actin network formation in vitro.
Study pointed-end capping and branch stability.
Analyze actin-driven membrane remodeling and trafficking mechanisms.
Format and handling
Supplied as a stable lyophilized protein complex for convenient storage and transport. After reconstitution, the preparation is intended for immediate use in actin nucleation, branching and cytoskeleton reconstitution workflows.
Hypermol Satisfaction Guarantee
Validated product quality with Satisfaction Guarantee.
Proteins from Hypermol® are of validated high biological activity, as described in our publications.
arp 23 complex 1 mg Hypermoll
Arp 2/3 protein complex - 2x50µg
Pointed end capping and F-actin branching protein, with a strong nucleation upon activation by VCA-domain. The seven-subunit protein complex comprises Arp2 and Arp3 and five smaller (Arc) proteins, which are all present in approximately equal stoichiometry.  
558,00 EUR
excl. Shipping costs
Arp 2/3 protein complex - 1.0 mg (2x500 µg)
Arp 2/3 protein complex - 1.0 mg (2x500 µg)
Pointed end capping and F-actin branching protein, with a strong nucleation upon activation by VCA-domain.    
5.022,00 EUR
excl. Shipping costs
vca_hwave1
VCA-GST (human recombinant WAVE VCA-domain, GST-tagged) - 250µg
Arp2/3 activating human WAVE VCA-domain (>95% purity).

 

243,00 EUR
excl. Shipping costs
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Arp2/3 Protein Complex: Essential for Actin Nucleation and Dynamics


High-Quality Arp2/3 Protein Complex

The **Arp2/3 Protein Complex** is a natively prepared seven-subunit assembly that plays a pivotal role in actin filament nucleation and branching. This highly purified and soluble protein complex is shipped in a stable lyophilized form, ensuring long-term shelf life and transport stability. Upon reconstitution, it becomes fully functional and ready-to-use for a variety of advanced research applications, including studies of actin dynamics and filament behavior.

The Arp2/3 complex enables precise analysis of actin cytoskeleton remodeling, making it ideal for cutting-edge techniques such as **TIRF microscopy (Total Internal Reflection Fluorescence)** and **high-resolution microscopy**. These advanced imaging methods allow researchers to visualize and quantify actin nucleation, branching, and network formation in real-time, providing unparalleled insights into cellular motility and structure.


Applications of Arp2/3 Protein Complex

  • Actin Dynamics Studies: Investigate actin nucleation, branching, and filament remodeling in vitro and in cells
  • High-Resolution Microscopy: Use in advanced imaging workflows such as TIRF microscopy for real-time visualization
  • Cellular and Molecular Research: Study cell motility, endocytosis, and cytoskeletal organization

Why Choose Arp2/3 Protein Complex from Hypermol?

  • Natively prepared seven-subunit complex for optimal biological activity
  • Highly purified and free from contaminants for reliable and reproducible results
  • Supplied in a stable lyophilized format for long-term storage and transport
  • Fully functional upon reconstitution and ready for immediate use


Enhance Your Research with Arp2/3 Protein Complex

The Arp2/3 Protein Complex is a trusted tool for researchers exploring cytoskeletal organization, actin filament dynamics, and cellular behavior. Its compatibility with advanced imaging techniques such as TIRF microscopy and other high-resolution microscopy makes it indispensable for studying real-time actin remodeling and branching mechanisms.
Contact us today to learn more or place an order.