Buffers & Staining kits


Fresh buffers are the only solutions for high protein performance!
Suitable buffers are vital for your proteins.




Buffers from Hypermol are made from analytical grade chemicals in ultrapure water, to meet your high demands. 

Here you find ready-to-use buffer with components of short shelf-life (ATP, DTT) for optimal conditions. As an example, the actin polymerization buffer PolyMix is supplied as a one step 10x stock solution: add 1ml of ultrapure water and mix 1:10 v/v with G-actin. PolyMix is used to dilute F-actin and ligands, providing conditions meeting the demands of in vitro works with actin filaments.


For high protein performance & convenient, time-saving bench work.  
 


 
Buffers from Hypermol are made of purest reagents in Milli-Q™ water, as described in our publications. 

Validated product quality with Satisfaction Guarantee! 

 






 
 
GTP | 100mM, pH 7.4
GTP | 100mM, pH 7.4
Add 1.0ml of H2O to obtain a fresh 100mM stock solution. 
Powder: 100mM ATP, 5.0mM Tris-Cl, pH 7.4. 
42,00 EUR
excl. Shipping costs
GTPγS | 100mM, pH 7.4
GTPγS | 100mM, pH 7.4
Add 25 µl of H2O to obtain a fresh 100mM stock solution. 
Powder: 100mM GTPγS, 50 mM Hepes titrated with NaOH to pH 7.4. 
69,00 EUR
excl. Shipping costs
ATP Actin Buffer , 1L (10x G-Actin buffer )
ATP Actin Buffer , 1L (10x G-Actin buffer )
ATP Actin Buffer is a G-actin buffer to dialyse G-actin and/or G-actin ligands. 

Essential G-actin buffer to:
 
  • maintains the G-actin state;
  • used for dialysis of actin and ligands in actin assays;
from 182,00 EUR
excl. Shipping costs
ADP | 100mM, pH 7.4
ADP | 100mM, pH 7.4
Add 1.0 ml of H2O to obtain a fresh 100mM stock solution. 
Powder: 100mM ADP, 20 mM Hepes titrated with NaOH to pH 7.4. 
37,00 EUR
excl. Shipping costs
KMEI Buffer (10x) - 2x1.0ml
KMEI Buffer (10x) - 2x1.0ml
Multipurpose buffer to:
  • use for many cytoskeletal proteins;
  • to polymerize actin;
from 28,00 EUR
excl. Shipping costs
AMP | 100mM, pH 7.4
AMP | 100mM, pH 7.4
Add 1.0 ml of H2O to obtain a fresh 100mM stock solution. 
Powder: 100mM AMP, 20 mM Hepes titrated with NaOH to pH 7.4. 
28,00 EUR
excl. Shipping costs
CS-LB (Cytoskeleton LysisBuffer)
CS-LB (Cytoskeleton LysisBuffer)
CS-LB is used to:
  • prepare cell lysates for cytoskeleton research;
  • support the stability of cytoskeletal proteins;
  • prevent proteolytic degradation; 
from 138,00 EUR
excl. Shipping costs
CS-LSB (Cytoskeleton Low Salt Buffer)
CS-LSB (Cytoskeleton Low Salt Buffer)
CS-LSB is used to:
  • Washing and low salt buffering of cell lysate derived preparations;
from 89,00 EUR
excl. Shipping costs
CS-MSB (Cytoskeleton Medium Salt Buffer)
CS-MSB (Cytoskeleton Medium Salt Buffer)
CS-MSB is used to:
  • washing and buffer cell lysate derived preparations;
  • support the stability of cytoskeletal proteins; 
from 89,00 EUR
excl. Shipping costs
FAB-P
FAB-P
FAB-P is used for Biacore SPR to:
  • support ligand binding to F-actin;
  • maintain the filamentous state of actin;
  • prevent denaturing of actin;
62,00 EUR
excl. Shipping costs
GAB-P
GAB-P
GAB-P is used for Biacore SPR to:
  • support ligand binding to G-actin;
  • maintain the G-state of actin;
  • prevent denaturing of actin;
51,00 EUR
excl. Shipping costs
CryoProtect plus DTT (liquid protein freezing medium) -10ml
CryoProtect plus DTT (liquid protein freezing medium) -10ml
CryoProtect is used to store soluble proteins at -20°C, with maximum preservation of biological activity. Ready-to-use. 
from 71,00 EUR
excl. Shipping costs
PolyMix 6 (actin polymerization buffer, 1ml -10x)
PolyMix 6 (actin polymerization buffer, 1ml -10x)
PolyMix 6 is used to:
  • facilitate ligand binding to F-actin at pH 6.0;
  • polymerize G-actin to F-actin;
  • support the F-actin state;
  • prevent denaturing of actin; 
from 35,00 EUR
excl. Shipping costs
ATPγS | 100mM, pH 7.4
ATPγS | 100mM, pH 7.4
Add 25 µl of H2O to obtain a fresh 100mM stock solution. 
Powder: 100mM GTPγS, 50 mM Hepes titrated with NaOH to pH 7.4. 
69,00 EUR
excl. Shipping costs
MotorMix II (for Myosin II ) - 100ml
MotorMix II (for Myosin II ) - 100ml
High ionic strength buffer for soluble myosin II. DTT and ATP are additionally provided with the buffer to be freshly added before use.
89,00 EUR
excl. Shipping costs
MotorMix I (for Myosin II filaments) - 100ml
MotorMix I (for Myosin II filaments) - 100ml
Physiological buffer to maintain or induce myosin II filaments. DTT and ATP are provided with the buffer to be freshly added before use. 
86,00 EUR
excl. Shipping costs
PolyMix 8 (actin polymerization buffer, 1ml -10x)
PolyMix 8 (actin polymerization buffer, 1ml -10x)
PolyMix 8 is used to:
  • facilitate ligand binding to F-actin at pH 8.0;
  • polymerize G-actin to F-actin;
  • support the F-actin state;
  • prevent denaturing of actin; 
from 35,00 EUR
excl. Shipping costs
CS-HSB (Cytoskeleton High Salt Buffer)
CS-HSB (Cytoskeleton High Salt Buffer)
CS-HSB is used to:
  • treatment of cell lysate derived preparations under highly stringent conditions;
from 104,00 EUR
excl. Shipping costs
CryoProtect HS plus DTT (liquid freezing medium) - 10ml
CryoProtect HS plus DTT (liquid freezing medium) - 10ml
CryoProtect is used for long-term storage of myosin and NEM-Myosin at -20°C, with maximum preservation of biological activity. Ready-to-use. 
from 77,00 EUR
excl. Shipping costs
ATP-Sucrose Cushion
ATP-Sucrose Cushion
SucroseCushions prevent non-specific cosedimentation of proteins, which were not bound to F-actin in high-speed centrifugation assays. 
34,00 EUR
excl. Shipping costs
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