
GAB-P
10x G-Actin buffer with Surfactant P-20 for SPR (liquid)
Cat. #: 5230-01
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Description
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Product name
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GAB-P
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Product description
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GAB-P is used for G-Actin in SPR techniques. The buffer maintains the structural integrity of G-actin and prevents from denaturing, GAB-P can be used dilution and preparation of G-actin and ligand samples, which require G-actin conditions. The buffer contains all components essential for the biological activity of G-actin.
GAB-P is a sterile filtered and degased 10x solution, that is supplemented with ATP & DTT (included as separate components). After the addition of ATP & DTT the pH is checked or adjusted to pH 8.2 and finally degassed.
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Overview
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GAB-P is used to:
- support ligand binding to G-actin;
- maintain the G-state of actin;
- prevent denaturing of actin;
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Properties
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Form
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Liquid, ready-to-use.
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Quantity
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1x25 ml (10x stock solution to make 250ml)
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Buffer
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0.4mM ATP, 0.08mM CaCl2., 0.0005% Surfactant P20, Buffer: 2mM Tris pH 8.2.
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Purity
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Ultrapure reagents in Milli-Q™
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Storage instructions
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GAB-P is stored at -20°C upon arrival and will be stable in performance for 3 months from the date of shipping. Prepare aliquots and avoid refreezing.
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Shipping conditions
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At ambient temperature. Upon delivery store at -20°C.
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Remarks
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For Use in Research only. Not for Use in Human or Veterinary Diagnostical or Therapeutical Applications.
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CAS no.
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Further Information
Product DataSheet
Material and Safety Data Sheet
Buffers from Hypermol are made of purest reagents in Milli-Q™
water, as described in our publications.
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