Actin-Toolkit Fluorometry

 
Actin-Toolkit Fluorometry
Actin kinetics
Polymerization & depolymerization
Ready-to-use assay system
 
Fluorometric analysis of actin polymerization and depolymerization
Real-time analysis of actin dynamics
Fluorescence-based kinetic measurements provide continuous readout of actin polymerization and depolymerization in solution.
Quantitative actin dynamics

Quantitative analysis of actin polymerization and depolymerization

Ready-to-use fluorometric assay systems for kinetic analysis of actin dynamics and their modulation by proteins, extracts, lipids and pharmacological compounds.

The Hypermol® Actin-Toolkit Fluorometry provides a defined experimental system for monitoring actin polymerization and depolymerization by fluorescence in solution. Fluorescent actin serves as the kinetic reporter, allowing changes in filament assembly and disassembly to be followed continuously over time.

The toolkits are designed for analysis of purified native or recombinant proteins, protein fragments, lipids and other actin-modulating ligands, as well as cell or tissue extracts. In addition to polymerization and depolymerization kinetics, the assay format can be used to investigate nucleation, sensitivity to divalent cations or pharmacological compounds, and F-actin severing effects under steady-state conditions.

Kinetic analysis
Continuous fluorescence monitoring of actin polymerization and depolymerization.
Actin modulation
For proteins, fragments, lipids, pharmacological compounds and complex biological extracts.
Functional readouts
Polymerization, depolymerization, nucleation and steady-state F-actin severing.
Browse Fluorometry Toolkits
Fluorescent actin readouts
The Fluorometry series includes Pyrene Actin as well as fluorescent Actin488, Actin550 and Actin647, providing alternative spectral readouts for kinetic measurements of actin dynamics.
Controlled assay conditions
Included actin buffer components, PolyMix and CaCl2 support defined polymerization conditions and experimental variation of ionic parameters.
Typical applications
Polymerization and depolymerization kinetics, characterization of actin-binding and actin-regulatory proteins, analysis of mutants or protein fragments, pharmacological modulation, divalent-cation dependence, cell- and tissue-extract analysis, nucleation studies and F-actin severing assays.
Pyrene Actin principle
Pyrene-labelled actin provides a highly responsive fluorescence readout of the transition from G-actin to F-actin and is established for quantitative kinetic measurements in solution.
Hypermol Actin-Toolkit Fluorometry
All-in-one experimental system.
The toolkit combines fluorescent actin with assay components and a detailed experimental handbook to support standardized preparation, polymerization and depolymerization workflows.
Hypermol Satisfaction Guarantee
Validated product quality with Satisfaction Guarantee.
Actin products from Hypermol® are prepared and quality controlled for reliable biochemical and biophysical analysis of actin dynamics.
 
Content of Toolkit Fluorometry with Pyrene
Actin-Tookit Fluorometry - Pyrene Actin
λex 365m, λem 407nm

Blue fluorescent skeletal muscle α-actin (rabbit), DOL= 0.1, >99% pure.

  • Kinetic analysis of purified ligands, cell or tissue extracts on polymerization or depolymerization of actin;

  • Determination of sensitivity to divalent cations or pharmacological compounds during polymerization or depolymerization; 

  • Measurement of F-actin severing effects of purified ligands, cell or tissue extracts at steady-state;
     

764,00 EUR
excl. Shipping costs
Content of Toolkit Fluorometry with Actin488
Actin-Tookit Fluorometry - Actin488
λex 495m, λem 520nm

Green fluorescent skeletal muscle α-actin (rabbit), DOL= 0.1, >99% pure.

  • Kinetic analysis of purified ligands, cell or tissue extracts on polymerization or depolymerization of actin;

  • Determination of sensitivity to divalent cations or pharmacological compounds during polymerization or depolymerization; 

  • Measurement of F-actin severing effects of purified ligands, cell or tissue extracts at steady-state;
     

794,00 EUR
excl. Shipping costs
Toolkit Fluorometry Actin ATTO 550
Actin-Tookit Fluorometry - Actin550
λex 550m, λem 570nm

Yellow fluorescent skeletal muscle α-actin (rabbit), DOL= 0.1, >99% pure.

  • Kinetic analysis of purified ligands, cell or tissue extracts on polymerization or depolymerization of actin;

  • Determination of sensitivity to divalent cations or pharmacological compounds during polymerization or depolymerization; 

  • Measurement of F-actin severing effects of purified ligands, cell or tissue extracts at steady-state;
     

794,00 EUR
excl. Shipping costs
Toolkit Fluorometry Actin Atto 647
Actin-Tookit Fluorometry - Actin647
λex 645m, λem 670nm

Red fluorescent skeletal muscle α-actin (rabbit), DOL= 0.1, >99% pure.

  • Kinetic analysis of purified ligands, cell or tissue extracts on polymerization or depolymerization of actin;

  • Determination of sensitivity to divalent cations or pharmacological compounds during polymerization or depolymerization; 

  • Measurement of F-actin severing effects of purified ligands, cell or tissue extracts at steady-state;
     

794,00 EUR
excl. Shipping costs
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