Pyrene Actin
Pyrene Actin
Actin polymerization kinetics
Cys374-labeled G-actin
100% and 10% pyrene formats
Typical applications
Fluorometry, fluorimetry, pyrene-actin assays, polymerization kinetics, depolymerization kinetics, nucleation assays and ligand-dependent actin dynamics.
Product focus
Pyrene-labeled actin at defined dye/protein ratios for sensitive, quantitative and reproducible analysis of actin filament dynamics.
Validated product quality with Satisfaction Guarantee.
Proteins from Hypermol® are of validated high biological activity, as described in our publications.
Proteins from Hypermol® are of validated high biological activity, as described in our publications.
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Introduction to Fluorometry is Actin
Pyrene Actin to measure actin polymerisation and depolymerisation
G-actin (globular actin) is a monomeric protein, which is the subunit of the actin microfilaments. Since G-actin polymerizes reversibly into double-helical microfilaments (the "actin helix"), it constitutes the "actin-based cytoskeleton" of practically all eucaryotic cells. Actin became a key player for cellular movement and force exercise (muscle, cell division). This arose only by the ability of hundreds of proteins with highly indidviual capabilities to evolve binding sites for actin and thus to utilize the actin filaments to assemble, and disassemble multi-protein-complex suprastructures needed for the motile and scaffolding functions of living cells.
To characterize actin-binding proteins, but also drugs / natural compounds / non-protein ligands in their abilty to modify the polymerization or depolymerization of actin, the actin kinetics are measured by fluorometry. This method requires Pyrene-Actin, which is chemically modified G-actin, carrying a pyrenyl group covalently bound to Cys374 at the C-term of the molecule.
The actin polymerization can be induced simply by mono- or divalent cations (in-vitro conditions). and the actin kinetic can then be measured fluorometrically. Upon excitation a characteristic kinetic is observed, which instantly shows the effect of ligands on the assembly or disassembly of the actin filaments.
Pyrene Actin to measure actin polymerisation and depolymerisation
G-actin (globular actin) is a monomeric protein, which is the subunit of the actin microfilaments. Since G-actin polymerizes reversibly into double-helical microfilaments (the "actin helix"), it constitutes the "actin-based cytoskeleton" of practically all eucaryotic cells. Actin became a key player for cellular movement and force exercise (muscle, cell division). This arose only by the ability of hundreds of proteins with highly indidviual capabilities to evolve binding sites for actin and thus to utilize the actin filaments to assemble, and disassemble multi-protein-complex suprastructures needed for the motile and scaffolding functions of living cells.
To characterize actin-binding proteins, but also drugs / natural compounds / non-protein ligands in their abilty to modify the polymerization or depolymerization of actin, the actin kinetics are measured by fluorometry. This method requires Pyrene-Actin, which is chemically modified G-actin, carrying a pyrenyl group covalently bound to Cys374 at the C-term of the molecule.
The actin polymerization can be induced simply by mono- or divalent cations (in-vitro conditions). and the actin kinetic can then be measured fluorometrically. Upon excitation a characteristic kinetic is observed, which instantly shows the effect of ligands on the assembly or disassembly of the actin filaments.