Pyrene Actin (100%)
α-actin from rabbit skeletal muscle
Cat. #: 8112-04
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Applications
Fluorimetrical Analysis of actin dynamics (e.g. kinetics of polymerization, nucleation, depolymerization)
Description
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Protein
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Pyrene Actin
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Origin
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Skeletal muscle alpha actin, rabbit
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Molecular mass
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42kDa
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Protein description
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G-actin from rabbit skeletal muscle is a single chain polypeptide with a molecular weight of 42kD consisting of 375 amino acids. Pyrene-Actin is chemically modified G-actin, carrying a pyrenyl group covalently bound to Cys374 at the C-term of the molecule.
The actin polymerization can be induced simply by mono- or divalent cations (in-vitro conditions) and the actin kinetic can then be measured fluorometrically. Fluorometry with Pyrenyl-Actin is standard method to study the effect of ligands on actin kinetics.
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Conjugation ratio
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Pyrene Actin (100%) is 100% labelled and thus has a dye/protein molar ratio of 1.
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Properties
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Form
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Lyophilized, ready-to-use.
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Quantity per unit
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4x250 µg
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Buffer
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2mM Tris-Cl pH 8.2, 0.4mM ATP, 0.5mM DTT, 0.1mM CaCl2, 1mM NaN3 and 0.3% disaccharides, when each tube is reconstituted with 250µl ultrapure water to obtain a 1mg/ml solution.
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Purity
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>99% by scanning densitometry.
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Purification notes
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Purified by GPC from rabbit skeletal muscle.
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Protein concentration
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Determined by the Biuret method.
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Storage instructions
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Pyrene actin is stored at –70°C upon arrival and will be stable in performance for at least 6 months from the date of purchase. Solubilized pyrene actin is kept on ice and should be used within 1 week. Avoid refreezing.
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Shipping conditions
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At ambient temperature. Upon delivery store at -70°C.
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Remarks
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CAS no.
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Further Information
Product DataSheet
Material and Safety Data Sheet
Protein Sequence on NCBI
References
Cysteine-rich protein 2 accelerates actin filament cluster formation.
Kihara T, Sugimoto Y, Shinohara S, Takaoka S, Miyake J.
PLoS One. 2017 Aug 16;12(8):e0183085. doi: 10.1371/journal.pone.0183085. eCollection 2017.