Protein

Actin (α-Actin)

Origin

Skeletal muscle alpha actin, rabbit

Molecular mass

42kDa

Protein description

Actin from rabbit skeletal muscle is a single chain polypeptide with a molecular weight of 42kD consisting of 375 amino acids.  It is characterized by binding to ATP/ADP (ATPase function) and the binding sites for divalent cations, that strongly contribute to the stabilization of actin.
Actin truly is a multi-ligand protein, which simply reflects its central importance as a major component of the cytoskeleton. The skeletal muscle actin (alpha-actin isoform) is highly conserved among vertebrates, which makes the rabbit skeletal muscle actin the most frequently used actin to investigate protein-protein interactions with cytoskeletal ligands.

The ability of actin monomers e.g. to polymerize into filaments of several µm lengths, to branch or perform the typical actin kinetics, strongly depends on the quality of the actin. Hypermol is manufacturing a premium quality actin. To provide the best actin we can possibly make, our proprietary freeze-drying technique preserves the highly functional actin, to arrive directly at your bench. 

Purity

>99% by scanning densitometry.

Form

Lyophilized, ready-to-use.

Quantity per unit

2x500 µg

Buffer

2mM Tris-Cl pH 8.2, 0.4mM ATP, 0.5mM DTT, 0.1mM CaCl2, 1mM NaN3 and 0.3% disaccharides, when reconstituted with 1.0 ml ultrapure water to obtain a 1mg/ml solution.

Related products

For dilution of G-actin or exchange of ligand buffer into actin compatible buffer, MonoMix (Cat. #.: 5100-0*) may be used. In the absence of nucleators, the polymerization of G-actin is initiated by PolyMix (Cat.#: 5000-0*). 

Purification notes

Purified from rabbit skeletal muscle, GPC.

Protein concentration

Determined by the Biuret method.

Storage instructions

Actin stored at –70°C upon arrival will be stable in performance for at least 6 months from the date of purchase. Solubilized G-actin is kept on ice and should be used within 1 week. Avoid refreezing.     

Shipping conditions 

At ambient temperature. Upon delivery store at -70°C.

Remarks

For Use in Research only. Not for Use in Human or Veterinary Diagnostical or Therapeutical Applications.

CAS no.

 51005-14-2