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Monomeric Actin (G-Actin)
freshly prepared from rabbit skeletal muscle α-actinCat. #: 8101-G
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Description
|
Protein |
Actin (α-Actin) - Actin monomers ( G-Actin) |
|
Origin |
Skeletal muscle alpha actin, rabbit |
|
Molecular mass |
42kDa |
|
Protein description |
Freshly prepared actin monomers are shipped on ice by overnight express, ready to start your assays tomorrow.
G-Actin prepared from rabbit skeletal muscle is a single chain polypeptide with a molecular weight of 42kD ( 375 amino acids). G-Actin from Hypermol is column purified (>99% purity) and pre-centrifuged at 100.000xg, 3h . |
|
Purity |
>99% by scanning densitometry. |
Properties
|
Form |
Liquid |
|
Quantity & Concentration |
2.0 mg ad 2 mg/ml (for other quantities see above). |
|
Buffer |
2mM Trizma, 1.0mM ATP, 0.1mM CaCl2. pH 8.2. |
|
Availability / Manufacturing time |
2-4 days. |
|
Purification notes |
Purified from rabbit skeletal muscle, GPC. |
|
Protein concentration |
Determined at A280 1mg/ml = 0.63 |
|
Storage instructions |
G-Actin is kept on ice and should be used within 1 week if kept on ice and avoidance of contamination. MonoMix (50ml) is the G-actin buffer , which is shipped with the product to dilute the actin or actin ligands. Do not freeze. |
|
Shipping conditions |
Wet ice |
|
Remarks |
For Use in Research only. Not for Use in Human or Veterinary Diagnostical or Therapeutical Applications. |
|
CAS no. |
51005-14-2 |
Further Information
Product DataSheet
Material and Safety Data Sheet
Protein Sequence on NCBI
References
Cosolvent and crowding effects on the polymerization kinetics of actin.
Rosin, Christopher, Paul Hendrik Schummel and Robert S. C. Winter.
Physical chemistry chemical physics : PCCP 17 13 (2015): 8330-7.
Exploring the stability limits of actin and its suprastructures.
Rosin C, Erlkamp M, Ecken JV, Raunser S, Winter R.
Biophys J. 2014 Dec 16;107(12):2982-2992. doi: 10.1016/j.bpj.2014.11.006.
Multivalent cross-linking of actin filaments and microtubules through the microtubule-associated protein Tau.
Cabrales Fontela Y, Kadavath H, Biernat J, Riedel D, Mandelkow E, Zweckstetter M.
Nat Commun. 2017 Dec 7;8(1):1981. doi: 10.1038/s41467-017-02230-8.
