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Alpha-Actinin

Alpha-Actinin 1 & 4

Actin cross-linking

Mechanosensitive networks

~200 kDa homodimers

Bundle. Cross-link. Sense force.

Native α-actinin proteins for actin filament architecture, mechanics and cell-dynamics studies.

Actin-binding cytoskeleton organizers

Alpha-Actinin proteins for actin bundling, mechanics and cell motility research

Native α-actinin 1 and α-actinin 4 for defined actin cross-linking, filament-network organization and mechanobiology-focused workflows.

Alpha-Actinins are homodimeric actin-binding proteins of the spectrin superfamily. Their antiparallel dimer architecture allows them to bridge neighboring actin filaments and build ordered bundles or mechanically reinforced networks.

Alpha-Actinin 1 is a robust tool for calcium-insensitive actin bundling and in vitro cytoskeleton reconstitution. Alpha-Actinin 4 adds a stronger cell-biology angle: it is widely used in studies of actin-network mechanics, focal adhesions, podocyte cytoskeleton, cell spreading, migration and invasion. Together, the two isoforms support experiments from clean biochemical bundling assays to disease-relevant mechanobiology.

Alpha-Actinin 1

Best for defined actin bundling, filament spacing and calcium-insensitive cross-linking assays.

Alpha-Actinin 4

Strong choice for actin mechanics, focal adhesion maturation, migration and podocyte/cell-shape studies.

Network control

Use to tune filament bundling, cross-link density and mechanical reinforcement in actin systems.

Browse Alpha-Actinin proteins

For actin-bundling assays

Use α-actinin to convert individual actin filaments into defined bundles and to study how protein concentration, ionic conditions and filament density shape network architecture.

For mechanobiology

Alpha-Actinin 4 is especially relevant where actin networks transmit force, reinforce cortex-like structures or connect cytoskeletal architecture to adhesion and cell movement.

For disease-oriented cell biology

ACTN4 is strongly linked to podocyte cytoskeleton integrity and cancer cell motility/invasiveness, making α-actinin 4 useful beyond basic actin biochemistry.

Strong experimental use cases

Build defined actin bundles for in vitro reconstitution.

Compare α-actinin 1 and α-actinin 4 in network mechanics.

Probe focal adhesion and stress-fiber maturation pathways.

Model podocyte cytoskeleton defects and cell spreading behavior.

Study actin-network reinforcement under mechanical load.

Investigate migration, invasion and cytoskeletal remodeling.

Validated product quality with Satisfaction Guarantee.
Proteins from Hypermol^® are of validated high biological activity, as described in our publications.

Alpha-Actinin 1 (smooth muscle) - 2x 100 µg

Actin cross linking protein from smooth muscle, ~97% purity.

210,00 EUR

excl. Shipping costs

Alpha-Actinin (smooth muscle) - 1.0 mg

Actin cross linking protein from smooth muscle, ~97% purity.

from 1.680,00 EUR

excl. Shipping costs

Alpha-Actinin 4 (non-muscle) - 2x50 µg

Alpha Actinin 4 (non-muscle α-Actinin) has a molecular weight of ~200 kDa and functions as antiparallel homodimer, so that the N-terminal lateral domains can effectively cross-link actin filaments. Purity: ~98%.

287,00 EUR

excl. Shipping costs

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