
α-Actinin 4
(non-muscle isoform)
Cat. #: 8311-01
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Description
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Protein
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α-Actinin 4 (non-muscle)
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Origin
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murine, recombinant
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Molecular mass
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~100 kDa each monomer / ~200kDa per dimer
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Protein description
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α-Actinins belong to the spectrin superfamily and are divided in four isoforms, encoded by different genes. Isoform 2 and 3 – the so called "muscle" α-actinins are constituents of sarcomeres in the cross-striated skeletal and cardiac muscle. Isoform 1 and 4 are "non-muscle" α-actinins and present in practically every tissue.
α-Actinin 4 has a molecular weight of ~100 kDa and functions as antiparallel homodimer, so that the N-terminal lateral domains can effectively bundle actin filaments. α-Actinin 4 is ubiquitously expressed in normal non-muscle cells and neoplastic tissue, albeit the degrees vary. All spectrin-related proteins share a similar structure: 1) an N-terminal actin-binding site formed by a pair of calponin-homology (CH) domains; 2) a central rod domain consisting of variable numbers of spectrin-like repeats, serving as docking sites of cytosolic and nuclear proteins; 3) a calmodulin domain with two EF-hand motifs at the C-ter.
α-Actinin 4 predominantly localizes in the cytoplasm, while it likewise has been detected in the nucleus of some cells. Activation of intracellular biochemical pathways or actin filament depolymerization may trigger α-Actinin 4 to translocate to the nucleus.
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Actin interaction
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Actin cross linking protein. Kd actin-binding = 1-5µM.
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Properties
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Form
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Lyophilized, ready-to-use.
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Quantity per unit
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2x50 µg
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Buffer
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100mM NaCl, 20mM Tris-cl pH 7.4, 0.5mM DTT and 3% disaccharides, when reconstituted with 50µl ultrapure water to obtain a 1mg/ml solution.
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Purity
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>98% by scanning densitometry from Coomassie G-250 stained SDS-Gels.
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Purification notes
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Purified from turkey gizzard muscle (IEX, SEC).
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Protein concentration
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Determined UV-VIS (A280=0.1201)
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Storage instructions
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Upon reconstitution the product is stored on ice. This product can also be stored as a glycerol stock at -20°C. Avoid repeated freeze / thaw cycles.
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Shipping conditions
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At ambient temperature. Upon delivery store at -70°C.
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Remarks
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For Use in Research only. Not for Use in Human or Veterinary Diagnostical or Therapeutical Applications.
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CAS no.
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