Protein

α-Actinin 4 (non-muscle)

Origin

murine, recombinant

Molecular mass

~100 kDa each monomer / ~200kDa per dimer 

Protein description

α-Actinins belong to the spectrin superfamily and are divided in four isoforms, encoded by different genes. Isoform 2 and 3 – the so called "muscle" α-actinins are constituents of sarcomeres in the cross-striated skeletal and cardiac muscle. Isoform 1 and 4 are "non-muscle" α-actinins and present in practically every tissue.

α-Actinin 4 has a molecular weight of ~100 kDa and functions as antiparallel homodimer, so that the N-terminal lateral domains can effectively bundle actin filaments. α-Actinin 4 is ubiquitously expressed in normal non-muscle cells and neoplastic tissue, albeit the degrees vary. All spectrin-related proteins share a similar structure: 1) an N-terminal actin-binding site formed by a pair of calponin-homology (CH) domains; 2) a central rod domain consisting of variable numbers of spectrin-like repeats, serving as docking sites of cytosolic and nuclear proteins; 3) a calmodulin domain with two EF-hand motifs at the C-ter.
 
α-Actinin 4 predominantly localizes in the cytoplasm, while it likewise has been detected in the nucleus of some cells. Activation of intracellular biochemical pathways or actin filament depolymerization may trigger α-Actinin 4 to translocate to the nucleus.

Actin interaction

Actin cross linking protein. Kd actin-binding = 1-5µM.

Form

Lyophilized, ready-to-use.

Quantity per unit

2x50 µg

Buffer

100mM NaCl, 20mM Tris-cl pH 7.4, 0.5mM DTT and 3% disaccharides, when reconstituted with 50µl ultrapure water to obtain a 1mg/ml solution.

Purity

>98% by scanning densitometry from Coomassie G-250 stained SDS-Gels.

Purification notes

Purified from turkey gizzard muscle (IEX, SEC).

Protein concentration

Determined UV-VIS (A₂₈₀=0.1201)

Storage instructions

Upon reconstitution the product is stored on ice. This product can also be stored as a glycerol stock at -20°C. Avoid repeated freeze / thaw cycles.

Shipping conditions 

At ambient temperature. Upon delivery store at -70°C.

Remarks

For Use in Research only. Not for Use in Human or Veterinary Diagnostical or Therapeutical Applications. 

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