α-Actinin
(Actin Binding Protein)
| Protein | α-Actinin |
| Origin | Smooth muscle, turkey gizzard |
| Molecular mass | ~200 kDa |
| Protein description | Native α-actinin from smooth muscle has a molecular mass of ~200 kDa, as it exists as a homodimer of 2 × 100 kDa. Alpha-actinins belong to the spectrin superfamily and are divided into four isoforms encoded by different genes. Isoforms 2 and 3 (“muscle” α-actinins) are constituents of sarcomeres in cross-striated skeletal and cardiac muscle. Isoforms 1 and 4 (“non-muscle” α-actinins) are present in practically every tissue. |
| Actin interaction | Actin cross-linking protein. Kd (actin-binding) = 1–5 µM. |
| Form | Lyophilized, ready-to-use. |
| Quantity per unit | 1.0 mg |
| Buffer | 50 mM NaCl, 20 mM Tris pH 7.4, 0.5 mM DTT and 3% disaccharides (after reconstitution with 1 ml ultrapure water to obtain 1 mg/ml). |
| Purity | >97% by scanning densitometry from Coomassie G-250 stained SDS gels. |
| Purification notes | Purified from turkey gizzard muscle (IEX, SEC). |
| Protein concentration | Determined by UV-VIS (A280 = 0.1201). |
| Storage instructions | Upon reconstitution store on ice. Can also be stored as a glycerol stock at -20°C. Avoid repeated freeze/thaw cycles. |
| Shipping conditions | Ambient temperature. Upon delivery store at -70°C. |
| Remarks | For research use only. Not for use in human or veterinary diagnostic or therapeutic applications. |
| CAS no. | |
Proteins and buffers from Hypermol® are made of the ultrapure reagents in Milli-Q™ water, as described in our publications.
Reconstitution of contractile actomyosin rings in vesicles.
Litschel T, Kelley CF, Holz D, Adeli Koudehi M, Vogel SK, Burbaum L, Mizuno N, Vavylonis D, Schwille P. Nat Commun. 2021 Apr 15;12(1):2254. doi: 10.1038/s41467-021-22422-7.
Real-time dynamics of emerging actin networks in cell-mimicking compartments.
Deshpande S, Pfohl T. PLoS One. 2015 Mar 18;10(3):e0116521. doi: 10.1371/journal.pone.0116521.
A high molecular mass protein isolated from chicken gizzard: its localization at the dense plaques and dense bodies of smooth muscle and the Z-disks of skeletal muscle.
Terasaki AG, Nakagawa H, Kotani E, Mori H, Ohashi K. J Cell Sci. 1995 Mar;108 (Pt 3):857-68.
